Hughes J H, Biedenbach D J, Erwin M E, Jones R N
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
J Clin Microbiol. 1993 Dec;31(12):3255-9. doi: 10.1128/jcm.31.12.3255-3259.1993.
The E test (AB Biodisk, Solna, Sweden), a new approach developed to test antimicrobial susceptibility, was compared with the agar dilution method for seven-drug antibiogram analysis of Neisseria meningitidis isolates. The overall E-test quantitative accuracy (+/- 1 log2 dilution) was 93% compared with that of agar dilution testing. The E test was then used to perform the susceptibility tests on a 10-year sample of 102 N. meningitidis isolates, including 5 from a recent epidemic outbreak in the University of Iowa (Iowa City) community. The E test proved to be an efficient methodology for identifying common source clusters of meningococcal disease having resistance to rifampin or sulfonamides. Moreover, the data demonstrated a recent increase in penicillin MICs (MIC for 90% of strains, 0.094 microgram/ml) and an escalation of high-level resistance to trimethoprimsulfamethoxazole (33%) and rifampin (14%). The E test should be considered a simple and accurate susceptibility method for the emerging need to test meningococci and other pathogenic neisserias. Chocolate Mueller-Hinton agar was observed to provide the best support of growth and E-test MIC results that correlated well with results of the reference agar dilution method previously used for neisserias.
E试验（AB生物盘公司，瑞典索尔纳）是一种用于检测抗菌药物敏感性的新方法，该方法与琼脂稀释法用于脑膜炎奈瑟菌分离株的七药抗菌谱分析进行了比较。与琼脂稀释试验相比，E试验的总体定量准确性（±1个log2稀释度）为93%。随后，E试验被用于对102株脑膜炎奈瑟菌的10年样本进行药敏试验，其中包括来自爱荷华大学（爱荷华城）社区近期一次疫情暴发的5株菌株。E试验被证明是一种有效的方法，可用于识别对利福平或磺胺类药物耐药的脑膜炎球菌病共同来源聚集性病例。此外，数据显示青霉素的最低抑菌浓度（90%菌株的最低抑菌浓度为0.094微克/毫升）最近有所上升，对甲氧苄啶磺胺甲恶唑（33%）和利福平（14%）的高水平耐药性有所增加。鉴于对脑膜炎球菌和其他致病性奈瑟菌进行检测的新需求，E试验应被视为一种简单且准确的药敏方法。观察发现，巧克力穆勒-欣顿琼脂能为生长提供最佳支持，且E试验的最低抑菌浓度结果与先前用于奈瑟菌的参考琼脂稀释法结果高度相关。
