Mukwedeya D T, Takamatsu H, Parkhouse R M
Institute for Animal Health, Pirbright Laboratory, Woking, UK.
Vet Immunol Immunopathol. 1993 Nov;39(1-3):177-86. doi: 10.1016/0165-2427(93)90179-8.
All monoclonal antibodies (mAbs) submitted to the workshop panel were screened for reactivity with bovine surface immunoglobulin (sIg)+ cells (gated small dense lymphocytes from peripheral blood) by fluorescence activated cell sorter (FACS) analysis. Eighteen temporary clusters--TCs 1-12, 15, 16, 18, 19, 25 and 26--contained mAbs reactive with sIg+ cells. mAb BAS21A (unclustered) and CC92 (TC25) were also reactive with sIg+ cells. Further FACS analysis with B cells from peripheral blood lymphocytes and mesenteric lymph nodes, and B and T lymphoma cell lines, indicated that the majority of mAbs within TCs 2, 4, 15, 18 and 26 reacted specifically with bovine B cells. Bovine B cell specific mAbs within these clusters were TH14B, IL-A55, CACT101A, MUC76A from TC4, VPM30, GC65A, CACT65A from TC15, IL-A58, CC56, CC70, IL-A65 from TC18, and CC57 and 26A9 from TC26. Three mAbs--IL-A65, CC70, and BAQ15A--within TC18 defined WC3; mAbs TD9 and CC56 may also be related to WC3.
提交给研讨会小组的所有单克隆抗体（mAb）均通过荧光激活细胞分选仪（FACS）分析，筛选其与牛表面免疫球蛋白（sIg）阳性细胞（来自外周血的门控小而致密淋巴细胞）的反应性。18个临时簇——TCs 1 - 12、15、16、18、19、25和26——包含与sIg阳性细胞反应的单克隆抗体。单克隆抗体BAS21A（未聚类）和CC92（TC25）也与sIg阳性细胞反应。对外周血淋巴细胞和肠系膜淋巴结的B细胞以及B和T淋巴瘤细胞系进行的进一步FACS分析表明，TCs 2、4、15、18和26中的大多数单克隆抗体与牛B细胞特异性反应。这些簇中的牛B细胞特异性单克隆抗体分别为来自TC4的TH14B、IL - A55、CACT101A、MUC76A，来自TC15的VPM30、GC65A、CACT65A，来自TC18的IL - A58、CC56、CC70、IL - A65，以及来自TC26的CC57和26A9。TC18中的三种单克隆抗体——IL - A65、CC70和BAQ15A——定义了WC3；单克隆抗体TD9和CC56可能也与WC3相关。
