通过新型免疫层析法和乳胶凝集试验快速检测传染性单核细胞增多症相关嗜异性抗体。
Rapid detection of infectious mononucleosis-associated heterophile antibodies by a novel immunochromatographic assay and a latex agglutination test.
作者信息
Farhat S E, Finn S, Chua R, Smith B, Simor A E, George P, Diena B B, Diena D, Skulnick M
机构信息
Department of Microbiology, Daniel Medical Laboratories, Downsview, Ontario, Canada.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1597-600. doi: 10.1128/jcm.31.6.1597-1600.1993.
A novel immunochromatographic assay, the CARDS O.S. MONO test (Pacific Biotech, San Diego, Calif.), and a latex agglutination test, the Infectious Mononucleosis Kit (Unipath Ltd., Hampshire, United Kingdom) were compared with the Paul-Bunnell-Davidsohn test. Of the 957 serum specimens studied, 78 were positive and 879 were negative by the Paul-Bunnell-Davidsohn test. After discrepancies were resolved by determining Epstein-Barr virus serology, the sensitivities of the CARDS O.S. MONO test and the Infectious Mononucleosis Kit were 91.0 and 96.2%, respectively, and both tests had a specificity and a positive predictive value of 100% and a negative predictive value and overall agreement of greater than 99%. The results show that both tests can accurately detect infectious mononucleosis-associated heterophile antibodies.
将一种新型免疫层析检测法（CARDS O.S. MONO检测试剂盒，太平洋生物技术公司，加利福尼亚州圣地亚哥）和一种乳胶凝集试验（传染性单核细胞增多症检测试剂盒，Unipath有限公司，英国汉普郡）与嗜异性凝集试验进行了比较。在所研究的957份血清标本中，嗜异性凝集试验检测出78份阳性和879份阴性。在通过检测爱泼斯坦-巴尔病毒血清学解决差异后，CARDS O.S. MONO检测试剂盒和传染性单核细胞增多症检测试剂盒的灵敏度分别为91.0%和96.2%，两种检测方法的特异性和阳性预测值均为100%，阴性预测值和总体一致性均大于99%。结果表明，两种检测方法都能准确检测出传染性单核细胞增多症相关的嗜异性抗体。
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