衣原体酶联免疫吸附测定检测阈值以下衣原体抗原的直接荧光抗体确认。
Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.
作者信息
Kellogg J A, Seiple J W, Stroll E S
机构信息
Immunology Laboratory, York Hospital, Pennsylvania 17405.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1646-7. doi: 10.1128/jcm.31.6.1646-1647.1993.
Abstract
Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed with the direct fluorescent-antibody test (Syva) from among those 66 Chlamydiazyme-negative specimens which had A492s that ranged from 0.030 to the cutoff and that could be blocked by > or = 50% with the blocking reagent.
摘要
在使用衣原体酶联免疫吸附测定法（雅培实验室）检测的4000份宫颈内膜标本中，233份（5.8%）结果呈阳性（A492高于临界值），这些结果通过一种封闭试剂（雅培）得到确认。在66份衣原体酶联免疫吸附测定法结果为阴性、A492值在0.030至临界值之间且能被封闭试剂封闭≥50%的标本中，另外检测到34份（14.6%）含有衣原体抗原的标本，并通过直接荧光抗体检测法（赛瓦）得到确认。
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本文引用的文献
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