Stockley E W, Cole H M, Brown A D, Wheal H V
Department of Physiology and Pharmacology, University of Southampton, UK.
J Neurosci Methods. 1993 Apr;47(1-2):39-51. doi: 10.1016/0165-0270(93)90020-r.
A system for accurately reconstructing neurones from optical sections taken at high magnification is described. Cells are digitised on a 68000-based microcomputer to form a database consisting of a series of linked nodes each consisting of x, y, z coordinates and an estimate of dendritic diameter. This database is used to generate three-dimensional (3-D) displays of the neurone and allows quantitative analysis of the cell volume, surface area and dendritic length. Images of the cell can be manipulated locally or transferred to an IBM 3090 mainframe where a wireframe model can be displayed on an IBM 5080 graphics terminal and rotated interactively in real time, allowing visualisation of the cell from all angles. Space-filling models can also be produced. Reconstructions can also provide morphological data for passive electrical simulations of hippocampal pyramidal cells.
描述了一种用于从高倍光学切片精确重建神经元的系统。细胞在基于68000的微型计算机上进行数字化处理，以形成一个数据库，该数据库由一系列相互连接的节点组成，每个节点包含x、y、z坐标以及树突直径的估计值。该数据库用于生成神经元的三维（3-D）显示，并允许对细胞体积、表面积和树突长度进行定量分析。细胞图像可以在本地进行处理，也可以传输到IBM 3090大型主机上，在那里可以在IBM 5080图形终端上显示线框模型，并实时进行交互式旋转，从而可以从各个角度可视化细胞。还可以生成实体填充模型。重建还可以为海马锥体细胞的被动电模拟提供形态学数据。
