Gerweck L E, Seneviratne T, Gerweck K K
Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Radiat Res. 1993 Jul;135(1):69-74.
The relationship between energy status and oxygen concentration was evaluated using Chinese hamster ovary cells over a gas-phase oxygen concentration ranging from 40 to 210,000 ppm O2. Energy status was examined in cells in complete medium equilibrated with specified gas-phase oxygen concentrations, in the presence and absence of glucose. The change in two parameters of energy status, adenylate energy charge, which is ([ATP] + 1/2[ADP])/([ATP] + [ADP] + [AMP]), and ATP/cell as a function of oxygen concentration, was compared with the radiation oxygen enhancement ratio. In the presence of glucose, neither parameter of energy status is substantially affected by oxygen at various concentrations. In the absence of glucose, energy status is affected over the oxygen concentration range which parallels the change in the radiation oxygen enhancement ratio. However, the magnitude of the changes in energy status is dependent upon the duration of oxygen deprivation. In addition, glucose deprivation alone results in a decrease in ATP/cell in the presence of 210,000 ppm O2.
使用中国仓鼠卵巢细胞，在气相氧浓度范围为40至210,000 ppm O₂的条件下，评估了能量状态与氧浓度之间的关系。在含有特定气相氧浓度且存在和不存在葡萄糖的完全培养基中，检测细胞的能量状态。将能量状态的两个参数（腺苷酸能量电荷，即([ATP] + 1/2[ADP])/([ATP] + [ADP] + [AMP])，以及作为氧浓度函数的ATP/细胞）的变化与辐射氧增强比进行了比较。在存在葡萄糖的情况下，能量状态的两个参数在不同浓度的氧下均未受到实质性影响。在不存在葡萄糖的情况下，能量状态在与辐射氧增强比变化平行的氧浓度范围内受到影响。然而，能量状态变化的幅度取决于缺氧的持续时间。此外，仅葡萄糖剥夺就会导致在210,000 ppm O₂存在时细胞内ATP含量的降低。
