van der Sanden B P, Rijken P F, Heerschap A, Bernsen H J, van der Kogel A J
Department of Radiology, University Hospital St Radboud, Nijmegen, The Netherlands.
Br J Cancer. 1997;75(10):1432-8. doi: 10.1038/bjc.1997.246.
The relationship between the bioenergetic status of human glioma xenografts in nude mice and morphometric parameters of the perfused vascular architecture was studied using (31)P magnetic resonance spectroscopy (MRS), fluorescence microscopy and two-dimensional digital image analysis. Two tumour lines with a different vascular architecture were used for this study. Intervascular distances and non-perfused area fractions varied greatly between tumours of the same line and tumours of different lines. The inorganic phosphate-nucleoside triphosphate (P(i)/NTP) ratio increased rapidly as mean intervascular distances increased from 100 microm to 300 microm. Two morphometric parameters - the percentage of intervascular distances larger than 200 microm (ivd200) and the non-perfused area fraction at a distance larger than 100 microm from a nearest perfused vessel (area100), - were deduced from these experiments and related to the P(i)/NTP ratio of the whole tumour. It is assumed that an aerobic to anaerobic transition influences the bioenergetic status, i.e. the P(i)/NTP ratio increased linearly with the percentage of ivd200 and the area100.
利用磷-31磁共振波谱（MRS）、荧光显微镜和二维数字图像分析技术，研究了裸鼠人胶质瘤异种移植瘤的生物能量状态与灌注血管结构形态学参数之间的关系。本研究使用了两种具有不同血管结构的肿瘤细胞系。同一细胞系的肿瘤与不同细胞系的肿瘤之间，血管间距离和非灌注面积分数差异很大。随着平均血管间距离从100微米增加到300微米，无机磷酸-核苷三磷酸（P(i)/NTP）比值迅速升高。从这些实验中推导出两个形态学参数——血管间距离大于200微米的百分比（ivd200）和距最近灌注血管大于100微米处的非灌注面积分数（area100），并将其与整个肿瘤的P(i)/NTP比值相关联。据推测，有氧向无氧的转变会影响生物能量状态，即P(i)/NTP比值随ivd200百分比和area100呈线性增加。
