广宿主范围不相容群P和Q质粒从大肠杆菌向伴放线放线杆菌的接合转移。
Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans.
作者信息
Goncharoff P, Yip J K, Wang H, Schreiner H C, Pai J A, Furgang D, Stevens R H, Figurski D H, Fine D H
机构信息
Division of Oral Infectious Diseases, School of Dental and Oral Surgery, Columbia University, New York, New York 10032.
出版信息
Infect Immun. 1993 Aug;61(8):3544-7. doi: 10.1128/iai.61.8.3544-3547.1993.
The first example of conjugal transfer of DNA from Escherichia coli to the periodontal pathogen Actinobacillus actinomycetemcomitans is presented. Derivatives of the incompatibility group P (IncP) plasmid RK2 successfully transferred from an E. coli donor to an A. actinomycetemcomitans recipient. The resulting A. actinomycetemcomitans transconjugants transferred the plasmids back to E. coli recipients. The IncP transfer functions were also used in trans to mobilize the IncQ plasmid pBK1 from E. coli to A. actinomycetemcomitans. The IncP and IncQ plasmids both transferred into A. actinomycetemcomitans at high frequencies (0.3 to 0.5 transconjugants per donor) and showed no gross deletions, insertions, or rearrangements. Determinations of MICs of various antibiotics for the A. actinomycetemcomitans transconjugant strains demonstrated the expression of ampicillin, chloramphenicol, and kanamycin resistance determinants.
本文展示了DNA从大肠杆菌向牙周病原体伴放线放线杆菌进行接合转移的首个实例。不相容群P（IncP）质粒RK2的衍生物成功地从大肠杆菌供体转移至伴放线放线杆菌受体。产生的伴放线放线杆菌接合子又将质粒回传给大肠杆菌受体。IncP转移功能还被用于反式动员IncQ质粒pBK1从大肠杆菌转移至伴放线放线杆菌。IncP和IncQ质粒均以高频率（每个供体产生0.3至0.5个接合子）转移至伴放线放线杆菌，且未出现明显的缺失、插入或重排。对伴放线放线杆菌接合子菌株各种抗生素的最低抑菌浓度测定表明其表达了氨苄青霉素、氯霉素和卡那霉素抗性决定簇。
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