Stotts R R, Namikoshi M, Haschek W M, Rinehart K L, Carmichael W W, Dahlem A M, Beasley V R
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
Toxicon. 1993 Jun;31(6):783-9. doi: 10.1016/0041-0101(93)90384-u.
A cyanobacterial (blue-green algal) bloom containing Microcystis aeruginosa (dominant), M. viridis, and M. wesenbergii, was collected from Homer Lake (Illinois, U.S.A.) in the summer of 1988 and microcystins were isolated. One microcystin of substantially reduced toxicity was isolated, together with ten hepatotoxic microcystins. The compound with reduced toxicity was nonlethal at 1 mg/kg (i.p. mouse) and was determined to have a (C3H7O2) mono-ester of the alpha-carboxyl on the Glu unit of microcystin-LR. The other nine microcystins apart from MCLR had approximate LD50S ranging from 97 micrograms/kg to 750 micrograms/kg.
1988年夏天，从美国伊利诺伊州荷马湖采集到一种含有铜绿微囊藻（占主导）、绿色微囊藻和惠氏微囊藻的蓝藻（蓝绿藻）水华，并从中分离出微囊藻毒素。分离出一种毒性大幅降低的微囊藻毒素，以及十种肝毒性微囊藻毒素。这种毒性降低的化合物在1毫克/千克（腹腔注射小鼠）时无致死性，经测定其为微囊藻毒素-LR的Glu单元上α-羧基的（C3H7O2）单酯。除微囊藻毒素-LR外的其他九种微囊藻毒素的半数致死剂量（LD50）约为97微克/千克至750微克/千克。
