Konradsen H B, Sørensen U B, Henrichsen J
Department of Bacteriology, Statens Seruminstitut, Copenhagen, Denmark.
J Immunol Methods. 1993 Aug 26;164(1):13-20. doi: 10.1016/0022-1759(93)90270-h.
We have developed an ELISA for antibody determination, superior to others hitherto described, in which optimal coating is achieved using phenylated pneumococcal capsular polysaccharides as coating antigen. The specificity of the assay is ensured by complete inhibition of antibodies against the species-specific pneumococcal antigen, C-polysaccharide (C-Ps). The method is sensitive, specific, reproducible, fast and easy to work with and can be used for both immunoglobulin class and subclass antibody determinations.
我们开发了一种用于抗体测定的酶联免疫吸附测定法（ELISA），该方法优于迄今所描述的其他方法，其中使用苯化肺炎球菌荚膜多糖作为包被抗原可实现最佳包被。通过完全抑制针对种特异性肺炎球菌抗原C多糖（C-Ps）的抗体来确保该测定法的特异性。该方法灵敏、特异、可重复、快速且易于操作，可用于免疫球蛋白类别和亚类抗体的测定。
