Amoako K K, Goto Y, Shinjo T
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
J Clin Microbiol. 1993 Aug;31(8):2244-7. doi: 10.1128/jcm.31.8.2244-2247.1993.
A total of 10 strains each of Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme were tested for the production of 13 extracellular enzymes. DNase, alkaline phosphatase, and lipase were predominantly associated with all the strains of F. necrophorum subsp. necrophorum, with DNase not detected in any of the strains of F. necrophorum subsp. funduliforme. In addition, the strains of F. necrophorum subsp. necrophorum were generally more hemolytic than those of F. necrophorum subsp. funduliforme. Lecithinase, beta-lactamase, elastase, hyaluronidase, chondroitin sulfatase, and coagulase were not detected in any of the strains. DNase may be used to differentiate between the two subspecies.
对坏死梭杆菌坏死亚种和坏死梭杆菌底形亚种各10株菌株进行了13种细胞外酶产生情况的检测。脱氧核糖核酸酶（DNase）、碱性磷酸酶和脂肪酶主要与坏死梭杆菌坏死亚种的所有菌株相关，而在坏死梭杆菌底形亚种的任何菌株中均未检测到DNase。此外，坏死梭杆菌坏死亚种的菌株通常比坏死梭杆菌底形亚种的菌株溶血活性更强。在任何菌株中均未检测到卵磷脂酶、β-内酰胺酶、弹性蛋白酶、透明质酸酶、硫酸软骨素酶和凝固酶。DNase可用于区分这两个亚种。
