Ward K N, Gray J J, Joslin M E, Sheldon M J
Department of Pathology, University of Cambridge, United Kingdom.
J Med Virol. 1993 Jan;39(1):44-9. doi: 10.1002/jmv.1890390109.
A newly developed IgG antibody avidity test for human herpesvirus-6 (HHV-6) was used in a study of primary and recurrent HHV-6 antibody responses in immunocompromised solid organ graft recipients. In a primary HHV-6 infection low avidity antibody was detected which matured to high avidity within 5 months whereas, in contrast, high avidity antibody was found in three recurrent infections thus showing the ability of the test to discriminate between primary and recurrent infection in immunosuppressed patients. Six HHV-6 seropositive transplant patients who experienced a subsequent primary human cytomegalovirus (CMV) infection had high avidity concurrent HHV-6 antibody rises, thus excluding a cross-reaction from the low avidity antibodies generated in the primary CMV response and providing evidence of recurrent HHV-6 infection. Four further HHV-6 seropositive patients with proven primary Epstein-Barr virus (EBV) infection were also studied; in each of these cases high avidity HHV-6 antibody rises were seen likewise suggesting recurrent HHV-6 infection. The test is therefore of value in the investigation of herpesvirus infections in transplant patients.
一种新开发的针对人类疱疹病毒6型（HHV-6）的IgG抗体亲和力检测方法，被用于一项针对免疫功能低下的实体器官移植受者中HHV-6原发性和复发性抗体反应的研究。在原发性HHV-6感染中，检测到低亲和力抗体，其在5个月内成熟为高亲和力抗体；而相比之下，在三次复发性感染中发现了高亲和力抗体，从而表明该检测方法能够区分免疫抑制患者的原发性感染和复发性感染。六名HHV-6血清阳性的移植患者随后发生了原发性人巨细胞病毒（CMV）感染，其HHV-6抗体同时出现高亲和力升高，从而排除了原发性CMV反应中产生的低亲和力抗体的交叉反应，并提供了HHV-6复发性感染的证据。另外对四名经证实发生原发性爱泼斯坦-巴尔病毒（EBV）感染的HHV-6血清阳性患者进行了研究；在每例病例中，同样观察到HHV-6抗体高亲和力升高，提示HHV-6复发性感染。因此，该检测方法在移植患者疱疹病毒感染的调查中具有重要价值。
