Smith M A, Braswell L D, Collins J G, Boyd D L, Jeffcoat M K, Reddy M, Li K L, Wilensky S, Vogel R, Alfano M
University of North Carolina, Chapel Hill 27599-7455.
Infect Immun. 1993 Apr;61(4):1453-9. doi: 10.1128/iai.61.4.1453-1459.1993.
Ligature-induced periodontitis was monitored for 6 months in eight Macaca mulatta monkeys to examine clinical status, radiographic bone level, and crevicular fluid (CF) levels of prostaglandin E2 (PGE2), thromboxane B2 (TxB2), interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha, and leukotriene B4 (LTB4). A split-mouth design was used, with eight ligated teeth and eight contralateral nonligated teeth which develop soft-chow-promoted (spontaneous) disease. Ligated sites experienced an average attachment loss of 0.94 mm per site and linear bone loss of 0.88 mm per site, with spontaneous-periodontitis sites experiencing approximately half the loss of ligated sites. The CF mediator levels showed increased levels of PGE2 and TxB2 at the ligated sites, as compared with the spontaneous sites, with no significant contralateral differences in the IL-1 beta or LTB4 responses. The concentrations of LTB4 in CF reached an early threefold peak over the baseline level at 1 month. By 2 months there was a statistically significant threefold elevation in CF-PGE2 in the ligated sites and a twofold elevation in the spontaneous sites as compared to the baseline level (P = 0.041 and 0.008, respectively). The monocyte product IL-1 beta increased sharply at 2 months and returned to the baseline level by 6 months at both ligated and nonligated sites. Tumor necrosis factor alpha in CF was below the limit of detection at all sites throughout the experiment (i.e., < 2 ng/ml). The selective elevation of both PGE2 and TxB2 in ligated sites, compared with levels in spontaneous sites, in the presence of similar levels of LTB4 and IL-1 beta provides further evidence that these molecules regulate the magnitude of the tissue-destructive response in progressive periodontitis.
在8只恒河猴中监测结扎诱导的牙周炎6个月，以检查临床状况、影像学骨水平以及龈沟液（CF）中前列腺素E2（PGE2）、血栓素B2（TxB2）、白细胞介素-1β（IL-1β）、肿瘤坏死因子α和白三烯B4（LTB4）的水平。采用双侧对照设计，8颗结扎牙和8颗对侧未结扎牙，后者发生软食促进（自发）疾病。结扎部位每个部位平均附着丧失0.94 mm，线性骨丧失0.88 mm，而自发牙周炎部位的丧失约为结扎部位的一半。与自发部位相比，结扎部位的CF介质水平显示PGE2和TxB2水平升高，IL-1β或LTB4反应在对侧无显著差异。CF中LTB4的浓度在1个月时比基线水平早期升高至三倍峰值。到2个月时，与基线水平相比，结扎部位的CF-PGE2有统计学意义的三倍升高，自发部位有两倍升高（分别为P = 0.041和0.008）。单核细胞产物IL-1β在2个月时急剧增加，在结扎和未结扎部位到6个月时均恢复到基线水平。在整个实验过程中，CF中的肿瘤坏死因子α在所有部位均低于检测限（即<2 ng/ml）。与自发部位相比，结扎部位PGE2和TxB2的选择性升高，同时LTB4和IL-1β水平相似，进一步证明这些分子调节进行性牙周炎中组织破坏反应的程度。
