克氏锥虫的核DNA和动基体DNA合成：用DNA聚合酶抑制剂进行的放射自显影研究
Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors.
作者信息
Rojas C, Gutierrez C, Litvak S, Solari A
机构信息
Departmento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
出版信息
Int J Parasitol. 1993 May;23(3):361-4. doi: 10.1016/0020-7519(93)90011-m.
PMID:8395479
Abstract
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase inhibitors (aphidicolin, aracytidine, and dideoxythimidine) were chosen to study the nuclear and kinetoplast DNA synthesis in vivo. Inhibition was mainly observed with the nuclear DNA.
摘要
通过将[3H]胸苷掺入克氏锥虫上鞭毛体形式的细胞核和动基体DNA中，利用放射自显影术研究了其DNA合成。两种DNA均被大量标记。选择三种真核DNA聚合酶抑制剂（阿非迪霉素、阿糖胞苷和双脱氧胸苷）来研究体内细胞核和动基体的DNA合成。主要观察到细胞核DNA受到抑制。
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