Berlin L E, Rorabaugh M L, Heldrich F, Roberts K, Doran T, Modlin J F
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland.
J Infect Dis. 1993 Oct;168(4):888-92. doi: 10.1093/infdis/168.4.888.
Standard virologic methods were used to characterize the relative contribution of each of the enterovirus classes to the etiology of aseptic meningitis during a prospective study of this disease among children < 24 months old. Viruses were isolated in cell culture from 164 (60%) of 274 cases identified over 5 years and in newborn mice from only 2 of 104 remaining cell culture-negative cases. Serologic tests identified the viral pathogen in 3 additional cases. The group B coxsackieviruses and the echoviruses were implicated in 156 (92%) of the 169 laboratory-diagnosed cases. Forty-eight percent of all diagnosed cases were due to group B coxsackievirus serotypes 2, 4, and 5; 78% of all cases were attributable to only 8 of the 67 known enterovirus serotypes. Polioviruses were the only viruses isolated from 7 children, including a cerebrospinal fluid isolate from 1 child and a urine isolate from another. Disease was attributable to the group A coxsackie-viruses for only 3 cases.
在一项针对24个月以下儿童的无菌性脑膜炎前瞻性研究中，采用标准病毒学方法来确定各类肠道病毒在该疾病病因中所起的相对作用。在5年时间里确诊的274例病例中，有164例（60%）的病毒在细胞培养中分离出来，在104例细胞培养阴性的剩余病例中，只有2例在新生小鼠中分离出病毒。血清学检测在另外3例病例中确定了病毒病原体。在169例实验室确诊病例中，B组柯萨奇病毒和埃可病毒占156例（92%）。所有确诊病例中有48%是由B组柯萨奇病毒2型、4型和5型引起的；所有病例中有78%仅归因于67种已知肠道病毒血清型中的8种。脊髓灰质炎病毒是从7名儿童中分离出的唯一病毒，其中1名儿童的脑脊液分离出病毒，另一名儿童的尿液分离出病毒。仅3例病例的病因可归因于A组柯萨奇病毒。
