Garbesi A, Capobianco M L, Colonna F P, Tondelli L, Arcamone F, Manzini G, Hilbers C W, Aelen J M, Blommers M J
Istituto ICoCEA-CNR, Ozzano Emilia (BO), Italy.
Nucleic Acids Res. 1993 Sep 11;21(18):4159-65. doi: 10.1093/nar/21.18.4159.
Unnatural L-2'-deoxyribonucleosides L-T, L-dC, L-dA and L-dG were prepared from L-arabinose and assembled, by solution or solid phase synthesis, to give L-oligonucleotides (L-DNAs), which contain all four natural bases. The affinity of these modified oligomers for complementary D-ribo- and D-deoxyribo-oligomers was studied with NMR, UV and CD spectroscopies and mobility shift assay on native PAGE. All experimental results indicate that L-DNAs do not, in general, recognize single-stranded, natural DNA and RNA. Hence, contrary to previous suggestions, it is not possible to envisage their use as wide scope antimessenger agents in the selective control of gene expression.
非天然的L-2'-脱氧核糖核苷L-T、L-dC、L-dA和L-dG由L-阿拉伯糖制备而成，并通过溶液或固相合成法组装成含有所有四种天然碱基的L-寡核苷酸（L-DNA）。利用核磁共振（NMR）、紫外（UV）和圆二色（CD）光谱以及天然聚丙烯酰胺凝胶电泳（native PAGE）上的迁移率变动分析，研究了这些修饰寡聚物与互补的D-核糖和D-脱氧核糖寡聚物的亲和力。所有实验结果表明，一般来说，L-DNA不能识别单链的天然DNA和RNA。因此，与之前的观点相反，无法设想将它们用作在基因表达的选择性控制中具有广泛应用范围的抗信使剂。
