Woods J P, Kersulyte D, Goldman W E, Berg D E
Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.
J Clin Microbiol. 1993 Feb;31(2):463-4. doi: 10.1128/jcm.31.2.463-464.1993.
The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day procedure traditionally used to extract DNA from H. capsulatum. We present here a quick (approximately 2-h) extraction method and show that the resultant DNA is suitable for sensitive and reproducible identification of individual strains of this pathogenic fungus.
任意引物聚合酶链反应(也称为随机扩增多态性DNA,即RAPD)是一种DNA指纹识别方法,它为鉴别荚膜组织胞浆菌的独立分离株提供了一种高效、灵敏的方式,但其广泛应用一直受到传统上用于从荚膜组织胞浆菌中提取DNA的耗时两天的繁琐程序的阻碍。我们在此介绍一种快速(约2小时)提取方法,并表明所得DNA适用于对这种致病真菌的各个菌株进行灵敏且可重复的鉴定。