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从荚膜组织胞浆菌中快速提取DNA：基于任意引物聚合酶链反应的流行病学和临床研究方法

Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies.

作者信息

Woods J P, Kersulyte D, Goldman W E, Berg D E

机构信息

Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.

出版信息

J Clin Microbiol. 1993 Feb;31(2):463-4. doi: 10.1128/jcm.31.2.463-464.1993.

DOI:10.1128/jcm.31.2.463-464.1993
PMID:8432840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262791/
Abstract

The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day procedure traditionally used to extract DNA from H. capsulatum. We present here a quick (approximately 2-h) extraction method and show that the resultant DNA is suitable for sensitive and reproducible identification of individual strains of this pathogenic fungus.

摘要

任意引物聚合酶链反应（也称为随机扩增多态性DNA，即RAPD）是一种DNA指纹识别方法，它为鉴别荚膜组织胞浆菌的独立分离株提供了一种高效、灵敏的方式，但其广泛应用一直受到传统上用于从荚膜组织胞浆菌中提取DNA的耗时两天的繁琐程序的阻碍。我们在此介绍一种快速（约2小时）提取方法，并表明所得DNA适用于对这种致病真菌的各个菌株进行灵敏且可重复的鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0026/262791/a8050a04f40b/jcm00014-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0026/262791/a8050a04f40b/jcm00014-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0026/262791/a8050a04f40b/jcm00014-0304-a.jpg

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本文引用的文献

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