Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
J Clin Microbiol. 2012 Jun;50(6):2159-62. doi: 10.1128/JCM.00450-12. Epub 2012 Mar 7.
This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.
本文描述了使用通用引物 T3B 进行 PCR 指纹图谱分析，以区分孢子丝菌复合体中的各个种，包括巴西副球孢子菌、球形孢子丝菌、墨西哥芽生菌和申克孢子丝菌。该方法产生了独特的带型模式，通过部分钙调蛋白（CAL）基因序列分析，可在种水平上正确鉴定所有 35 株临床分离株。该方法简单、可靠、快速且廉价，是临床真菌学实验室理想的常规鉴定系统。
