Kiagova A A, Zhuravel' N N, Belichenko I V, Khalidov E M, Potapenko A Ia
Biofizika. 1997 Jan-Feb;42(1):191-5.
Toxic effect of previously photooxidized in water psoralen on survival of mouse peritoneal exudate cells was investigated. Cell survival was determined by trypan exclusion test. It was shown that photooxidized psoralen itself did not induce trypan-positive cells while decrease in cell survival was observed in the case of simultaneous addition of photooxidized psoralen and Fe2+ ions to cell suspension. To evaluate the quantity of psoralen photoproducts which react with Fe2+ photooxidized psoralen Fe2+ mixtures were titrated by different cell concentrations. Then parameter c50 (cell concentration such that 50% trypan-positive cells were observed) was determined. It was shown that c50 increased with the increase in preirradiation fluence of psoralen from 4.5 to 45 kJ/m2. But further increase in preirradiation fluence resulted in decrease of c50. The photodestruction of psoralen photoproducts which react with Fe2+ ions was proposed. Simultaneous addition of photooxidized psoralen, Fe2+ ions and antioxidant butylated hydroxytoluene significantly increased cell survival. That indicated free radical nature of reaction products of photooxidized psoralen with Fe2+ ions.
研究了预先在水中光氧化的补骨脂素对小鼠腹腔渗出细胞存活的毒性作用。通过台盼蓝排斥试验测定细胞存活率。结果表明，光氧化的补骨脂素本身不会诱导台盼蓝阳性细胞，而在向细胞悬液中同时添加光氧化的补骨脂素和Fe2+离子时，观察到细胞存活率下降。为了评估与Fe2+反应的补骨脂素光产物的量，用不同的细胞浓度对光氧化的补骨脂素-Fe2+混合物进行滴定。然后测定参数c50（观察到50%台盼蓝阳性细胞的细胞浓度）。结果表明，随着补骨脂素预照射通量从4.5 kJ/m2增加到45 kJ/m2，c50增加。但预照射通量的进一步增加导致c50降低。提出了与Fe2+离子反应的补骨脂素光产物的光破坏作用。同时添加光氧化的补骨脂素、Fe2+离子和抗氧化剂丁基羟基甲苯可显著提高细胞存活率。这表明光氧化的补骨脂素与Fe2+离子反应产物具有自由基性质。
