Dube D K, Sherman M P, Saksena N K, Bryz-Gornia V, Mendelson J, Love J, Arnold C B, Spicer T, Dube S, Glaser J B
Department of Medicine, State University of New York Health Science Center, Syracuse 13210.
J Virol. 1993 Mar;67(3):1175-84. doi: 10.1128/JVI.67.3.1175-1184.1993.
DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by the polymerase chain reaction (PCR) with the primer pair SK110/SK111. This primer pair is conserved among the pol genes of all primate T-cell lymphoma viruses (PTLV) and flanks a 140-bp fragment of DNA which, when used in comparative analyses, reflects the relative degree of diversity among PTLV genomes. Cloning, sequencing, and phylogenetic comparisons of these amplified 140-bp pol fragments indicated that there are at least two distinct genetic substrains of HTLV-II in the Western Hemisphere. These data were confirmed for selected isolates by performing PCR, cloning, and sequencing with to 10 additional primer pair-probe sets specific for different regions throughout the PTLV genome. HTLV-II isolates from Seminole, Guaymi, and Tobas Indians belong in the new substrain of HTLV-II, while the prototype MoT isolate defines the original substrain. There was greater diversity among HTLV-II New World strains than among HTLV-I New World strains. In fact, the heterogeneity among HTLV-II strains from the Western Hemisphere was similar to that observed in HTLV-I and simian T-cell lymphoma/leukemia virus type I isolates from around the world, including Japan, Africa, and Papua New Guinea. Given these geographic and anthropological considerations and assuming similar mutation rates and selective forces among the PTLV, these data suggest either that HTLV-II has existed for a long time in the indigenous Amerindian population or that HTLV-II isolates introduced into the New World were more heterogeneous than the HTLV-I strains introduced into the New World.
采用引物对SK110/SK111，通过聚合酶链反应（PCR）成功扩增了17名感染人T细胞淋巴瘤/白血病病毒II型（HTLV-II）的不同个体外周血单个核细胞的DNA。该引物对在所有灵长类T细胞淋巴瘤病毒（PTLV）的pol基因中保守，侧翼为一段140bp的DNA片段，用于比较分析时，可反映PTLV基因组间的相对差异程度。对这些扩增的140bp pol片段进行克隆、测序和系统发育比较表明，在西半球至少存在两种不同的HTLV-II遗传亚株。通过使用另外10对针对PTLV基因组不同区域的引物-探针组进行PCR、克隆和测序，对选定的分离株证实了这些数据。来自塞米诺尔人、瓜伊米人和托巴斯印第安人的HTLV-II分离株属于HTLV-II的新亚株，而原型MoT分离株定义了原始亚株。HTLV-II新大陆毒株的多样性高于HTLV-I新大陆毒株。事实上，西半球HTLV-II毒株的异质性与在包括日本、非洲和巴布亚新几内亚在内的世界各地的HTLV-I和猿猴T细胞淋巴瘤/白血病病毒I型分离株中观察到的相似。考虑到这些地理和人类学因素，并假设PTLV之间的突变率和选择力相似，这些数据表明，要么HTLV-II在美洲印第安原住民中已存在很长时间，要么引入新大陆的HTLV-II分离株比引入新大陆的HTLV-I毒株更具异质性。
