乳酸克鲁维酵母着丝粒的共有序列与酿酒酵母功能性着丝粒DNA具有同源性。
The consensus sequence of Kluyveromyces lactis centromeres shows homology to functional centromeric DNA from Saccharomyces cerevisiae.
作者信息
Heus J J, Zonneveld B J, de Steensma H Y, van den Berg J A
机构信息
Leiden University, Department of Cell Biology and Genetics, The Netherlands.
出版信息
Mol Gen Genet. 1993 Jan;236(2-3):355-62. doi: 10.1007/BF00277133.
The nucleotide sequences of five of the six centromeres of the yeast Kluyveromyces lactis were determined. Mutual comparison of these sequences led to the following consensus: a short highly conserved box (5'-ATCACGTGA-3') flanked by an AT-rich (+/- 90%) stretch of +/- 160 bp followed by another conserved box (5'-TNNTTTATGTTTCCGAAAATTAATAT-3'). These three elements were named KlCDEI, KlCDEII, and KlCDEIII respectively, by analogy with the situation in Saccharomyces cerevisiae. In addition, a second 100 bp AT-rich (+/- 90%) element, named KlCDE0, was found +/- 150 bp upstream of KlCDEI. The sequences of both KlCDEI and KlCDEIII are highly conserved between K. lactis and S. cerevisiae; however, centromeres of K. lactis do not function in S. cerevisiae and vice versa. The most obvious differences between the centromeres of the two yeast species are the length of the AT-rich CDEII, which is 161-164 bp in K. lactis versus 78-86 bp in S. cerevisiae and the presence in K. lactis of KlCDE0, which is not found in S. cerevisiae.
测定了乳酸克鲁维酵母六个着丝粒中五个的核苷酸序列。这些序列的相互比较得出以下共有序列：一个短的高度保守框（5'-ATCACGTGA-3'），两侧是一段约160 bp、富含AT（约90%）的序列，后面接着另一个保守框（5'-TNNTTTATGTTTCCGAAAATTAATAT-3'）。根据酿酒酵母中的情况，这三个元件分别命名为KlCDEI、KlCDEII和KlCDEIII。此外，在KlCDEI上游约150 bp处发现了另一个100 bp富含AT（约90%）的元件，命名为KlCDE0。KlCDEI和KlCDEIII的序列在乳酸克鲁维酵母和酿酒酵母之间高度保守；然而，乳酸克鲁维酵母的着丝粒在酿酒酵母中不起作用，反之亦然。这两种酵母着丝粒之间最明显的差异是富含AT的CDEII的长度，在乳酸克鲁维酵母中为161 - 164 bp，而在酿酒酵母中为78 - 86 bp，以及乳酸克鲁维酵母中存在KlCDE0，而酿酒酵母中没有。
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