定量序列-活性模型（QSAM）——序列设计工具
Quantitative sequence-activity models (QSAM)--tools for sequence design.
作者信息
Jonsson J, Norberg T, Carlsson L, Gustafsson C, Wold S
机构信息
Department of Organic Chemistry, University of Umeå, Sweden.
出版信息
Nucleic Acids Res. 1993 Feb 11;21(3):733-9. doi: 10.1093/nar/21.3.733.
Abstract
Models have been developed that allow the biological activity of a DNA segment to be altered in a desired direction. Partial least squares projections to latent structures (PLS) was used to establish a quantitative model between a numerical description of 68 bp fragments of 25 E.coli promoters and their corresponding quantitative measure of in vivo strength. This quantitative sequence-activity model (QSAM) was used to generate two 68 bp fragments predicted to be more potent promoters than any of those on which the model originally was based. The optimized structures were experimentally verified to be strong promoters in vivo.
摘要
已经开发出了一些模型，这些模型能够使DNA片段的生物活性朝着预期的方向改变。偏最小二乘投影到潜在结构（PLS）被用于在25个大肠杆菌启动子的68bp片段的数值描述与其相应的体内强度定量测量之间建立定量模型。这个定量序列-活性模型（QSAM）被用于生成两个68bp片段，预测它们是比模型最初所基于的任何启动子都更强效的启动子。经实验验证，优化后的结构在体内是强启动子。
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