Wang Z Y, Hansen K, Sidén A, Cruz M
Department of Neurology, Karolinska Institute, Huddinge University Hospital, Sweden.
Scand J Immunol. 1993 Mar;37(3):369-76. doi: 10.1111/j.1365-3083.1993.tb02566.x.
Pre- and post-treatment (up to 3-26 months after antibiotic therapy) humoral immune responses were investigated in five neuroborreliosis patients. Anti-Borrelia (B.) burgdorferi IgG and IgM antibodies in CSF and serum were quantitated by capture ELISA. Agarose gel isoelectric focusing (AIF) and protein blotting were used to detect oligoclonal IgG and IgM bands as well as oligoclonal anti-B. burgdorferi IgG and IgM antibodies. These latter components were visualized by transfer to antigen-coated membranes (immunoblot) and immunoenzymatic staining. By ELISA, intrathecal anti-B. burgdorferi IgG and IgM antibody synthesis was detected in all initial specimens and continued 3-26 months after antibiotic therapy in four and three cases, respectively. AIF with protein blotting showed oligoclonal bands of total IgG as well as total IgM in the initial CSF specimens of all patients and persistence of such components occurred in four and five cases, respectively. By AIF and immunoblot, oligoclonal anti-B. burgdorferi IgG and IgM antibody bands could be detected in the CSF of every patient. IgG antibody bands were present in all initial CSF samples. The first specimen of one patient was negative for IgM antibody bands but such components appeared 3 weeks later. Oligoclonal CSF anti-B. burgdorferi IgG antibody components persisted over the entire follow-up periods in all but one case where they disappeared 6 weeks after treatment. The oligoclonal IgM antibodies in CSF vanished in two cases (after being present up to 4 and 11 months after antibiotic therapy) while they persisted over the entire (3-6 months after antibiotic therapy) follow-up periods in three cases. The specificity of the IgM antibody immunoblot technique was corroborated by control experiments, including antibody absorption studies and use of 41 kDa flagellar antigen.
对5例神经型莱姆病患者治疗前及治疗后（抗生素治疗后3 - 26个月）的体液免疫反应进行了研究。通过捕获ELISA对脑脊液和血清中的抗伯氏疏螺旋体IgG和IgM抗体进行定量。采用琼脂糖凝胶等电聚焦（AIF）和蛋白质印迹法检测寡克隆IgG和IgM条带以及寡克隆抗伯氏疏螺旋体IgG和IgM抗体。通过转移至抗原包被膜（免疫印迹）和免疫酶染色来观察这些成分。通过ELISA检测，在所有初始标本中均检测到鞘内抗伯氏疏螺旋体IgG和IgM抗体合成，抗生素治疗后4例和3例分别在3 - 26个月持续存在。AIF结合蛋白质印迹法显示，所有患者初始脑脊液标本中均存在总IgG和总IgM的寡克隆条带，4例和5例分别持续存在此类成分。通过AIF和免疫印迹法，在每位患者的脑脊液中均可检测到寡克隆抗伯氏疏螺旋体IgG和IgM抗体条带。所有初始脑脊液样本中均存在IgG抗体条带。1例患者的首个标本中IgM抗体条带为阴性，但3周后出现此类成分。除1例在治疗后6周消失外，所有患者脑脊液中寡克隆抗伯氏疏螺旋体IgG抗体成分在整个随访期间均持续存在。脑脊液中的寡克隆IgM抗体在2例中消失（抗生素治疗后分别存在4个月和11个月），而在3例中在整个随访期间（抗生素治疗后3 - 6个月）持续存在。包括抗体吸收研究和使用41 kDa鞭毛抗原在内的对照实验证实了IgM抗体免疫印迹技术的特异性。
