Osuga T, Mitamura K, Mashige F, Imai D
Clin Chim Acta. 1977 Feb 15;75(1):81-90. doi: 10.1016/0009-8981(77)90502-2.
Fasting serum bile acid (SBA) was measured by the enzymic fluorimetric method coupled with the NAD-resazurin system in 23 controls, 35 asymptomatic carriers of HIs antigen including 4 e antigen carriers and 91 patients with various liver diseases. All GHBs and e antigen carriers showed SBA within the normal range. SBA was most significantly correlated with serum bilirubin (gamma=0.74) and was a more sensitive index for impaired liver function than bilirubin or alkaline phosphatase in 164 radomly chosen samples from the liver disease group. In serial determinations of SBA with reference to GOT, GPT, changing patterns of these two parameters were classified into the parallel type and the discrepant type. Thirty two out of 40 cases with chronic liver disease belonged to the parallel type. SBA remained abnormal even after the normalization of transaminase in 12 out of 20 resolving episodes in cases of the parallel type, regardless of diagnosis. Since SBA changes according to the stage of the disease activity, serial and simultaneous estimation of SBA and GOT, GPT was found to be helpful in the observation of liver diseases. Maximum values of SBA elevation in an endogenous bile acid tolerance test after eating two egg yolks were higher than controls in 4 out of 7 cases with liver disease, who were associated with normal fasting SBA.
采用酶荧光法结合NAD - 刃天青系统，对23名对照者、35名乙肝表面抗原无症状携带者（包括4名e抗原携带者）以及91名各种肝病患者测定空腹血清胆汁酸（SBA）。所有乙肝表面抗原和e抗原携带者的SBA均在正常范围内。在从肝病组随机选取的164份样本中，SBA与血清胆红素的相关性最为显著（γ = 0.74），并且在反映肝功能损害方面，SBA比胆红素或碱性磷酸酶更为敏感。在参照谷草转氨酶（GOT）、谷丙转氨酶（GPT）对SBA进行系列测定时，这两个参数的变化模式可分为平行型和差异型。40例慢性肝病患者中有32例属于平行型。在平行型病例的20次病情缓解过程中，有12例即使转氨酶恢复正常后SBA仍异常，且与诊断无关。由于SBA随疾病活动阶段而变化，因此发现系列和同步测定SBA以及GOT、GPT有助于肝病的观察。7例肝病患者中有4例在进食两个蛋黄后进行的内源性胆汁酸耐量试验中SBA升高的最大值高于对照者，这些患者空腹SBA正常。
