Kobayashi K, Allen R M, Bloomer J R, Klatskin G
JAMA. 1979 May 11;241(19):2043-5.
Fasting serum total bile acid (SBA) levels were estimated by enzymatic fluorometry (EF) in 36 subjects without liver disease, 28 with hepatic lesions and impaired hepatic function, and 79 with hepatic lesions and normal function. Fasting and postprandial EF-SBA levels were compared in nine normal subjects and nine patients with cholestasis, and SBA assays by EF and gas-liquid chromatography (GLC) were compared in 28 patients with hepatic lesions and impaired function. Levels of SBA were below 9 mumole/L in all but two of the 36 subjects without liver disease, and above that level in all 28 with impaired hepatic function and 17 (24%) of the 70 with hepatic lesions and normal liver function. In most subjects, EF detected notable postprandial rises in SBA. Enzymatic fluorometric and GLC-SBA values were closely correlated (coefficient of correlation, 0.869).
采用酶荧光法（EF）测定了36例无肝脏疾病的受试者、28例有肝脏病变且肝功能受损的患者以及79例有肝脏病变但肝功能正常的患者的空腹血清总胆汁酸（SBA）水平。比较了9例正常受试者和9例胆汁淤积患者空腹及餐后的EF-SBA水平，并在28例有肝脏病变且肝功能受损的患者中比较了EF和气相色谱法（GLC）检测SBA的结果。36例无肝脏疾病的受试者中，除2例之外，其余所有人的SBA水平均低于9微摩尔/升；28例肝功能受损的患者以及70例有肝脏病变但肝功能正常的患者中，所有患者的SBA水平均高于该值。在大多数受试者中，EF检测到餐后SBA显著升高。酶荧光法和GLC-SBA值密切相关（相关系数为0.869）。
