Hunt A J, Connell J, Christofinis G, Parry J V, Weatherburn P, Hickson F C, Coxon A P, Davies P M, McManus T J, Sutherland S
Dulwich Hospital PHLS, London, UK.
Genitourin Med. 1993 Feb;69(1):29-30. doi: 10.1136/sti.69.1.29.
To assess the reliability of saliva samples as a means of testing for HIV-antibodies outside clinic settings.
Men taking part in a non-clinic longitudinal study of homosexually active men provided samples of saliva and blood. Sera were screened using a competitive ELISA (Wellcozyme) and positive sera were confirmed by an indirect ELISA (Abbott). Saliva samples were screened either using an IgG captive radioimmunoassay or an amplified ELISA.
A total of 534 paired saliva and blood samples were tested. Overall sensitivity was 96.2% and specificity was 100%. None of the saliva tests were falsely positive for HIV-1 antibodies.
HIV-1 saliva tests can reliably be used in a non-clinic or field setting. However, if results are to be given to respondents, it is necessary to offer adequate counselling and consider the mechanisms for referral and follow-up for those that are found to be HIV-1 antibody positive.
评估唾液样本作为在门诊环境以外检测HIV抗体手段的可靠性。
参与一项针对男同性恋活跃男性的非门诊纵向研究的男性提供了唾液和血液样本。血清采用竞争性酶联免疫吸附测定法（Wellcozyme）进行筛查，阳性血清通过间接酶联免疫吸附测定法（雅培）进行确认。唾液样本采用IgG捕获放射免疫测定法或扩增酶联免疫吸附测定法进行筛查。
共检测了534对唾液和血液样本。总体敏感性为96.2%，特异性为100%。所有唾液检测均未出现HIV-1抗体假阳性。
HIV-1唾液检测可在非门诊或现场环境中可靠使用。然而，如果要向受访者提供检测结果，则有必要提供充分的咨询，并考虑为那些被发现HIV-1抗体呈阳性的人提供转诊和随访机制。
