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核心结合因子的序列特异性

Sequence specificity of the core-binding factor.

作者信息

Melnikova I N, Crute B E, Wang S, Speck N A

机构信息

Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755.

出版信息

J Virol. 1993 Apr;67(4):2408-11. doi: 10.1128/JVI.67.4.2408-2411.1993.

DOI:10.1128/JVI.67.4.2408-2411.1993
PMID:8445737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240414/
Abstract

The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.

摘要

核心结合因子（CBF）可结合哺乳动物C型逆转录病毒增强子中的保守核心基序。我们通过乙基化干扰试验分析了CBF在莫洛尼氏鼠白血病病毒增强子上形成的磷酸接触。磷酸接触跨越围绕共有核心位点的9个碱基对。为了研究CBF结合的序列偏好性，我们采用了选择和扩增结合序列足迹法（T.K.布莱克韦尔和H.温特劳布，《科学》250:1104 - 1110，1990）。通过选择和扩增结合序列足迹法确定的CBF共有结合位点为PyGPyG GTPy。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43c9/240414/818f1814c875/jvirol00025-0678-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43c9/240414/818f1814c875/jvirol00025-0678-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43c9/240414/818f1814c875/jvirol00025-0678-a.jpg

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