Lois C, Alvarez-Buylla A
Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2074-7. doi: 10.1073/pnas.90.5.2074.
Subventricular zone (SVZ) cells proliferate spontaneously in vivo in the telencephalon of adult mammals. Several studies suggest that SVZ cells do not differentiate after mitosis into neurons or glia but die. In the present work, we show that SVZ cells labeled in the brains of adult mice with [3H]thymidine differentiate directly into neurons and glia in explant cultures. In vitro labeling with [3H]thymidine shows that 98% of the neurons that differentiate from the SVZ explants are derived from precursor cells that underwent their last division in vivo. This report identifies the SVZ cells as neuronal precursors in an adult mammalian brain.
室下区（SVZ）细胞在成年哺乳动物端脑的体内环境中自发增殖。多项研究表明，SVZ细胞在有丝分裂后不会分化为神经元或神经胶质细胞，而是死亡。在本研究中，我们发现，用[3H]胸腺嘧啶核苷标记成年小鼠大脑中的SVZ细胞后，这些细胞在体外培养物中可直接分化为神经元和神经胶质细胞。用[3H]胸腺嘧啶核苷进行体外标记显示，从SVZ外植体分化而来的神经元中，98%源自体内经历最后一次分裂的前体细胞。本报告将SVZ细胞鉴定为成年哺乳动物大脑中的神经元前体。
