Rayssiguier Y, Gueux E, Bussière L, Durlach J, Mazur A
INRA, Centre de Recherches en Nutrition Humaine, Theix, St-Genès-Champanelle, France.
J Am Coll Nutr. 1993 Apr;12(2):133-7. doi: 10.1080/07315724.1993.10718293.
Magnesium (Mg)-deficient and control diets were pair-fed to weanling Wistar rats for 8 days. Plasma lipoproteins were separated into various density classes by sequential preparative ultracentrifugation. The extent of lipid peroxidation was measured in terms of thiobarbituric acid reactive substances in lipoproteins and tissue homogenates before or after iron-induced lipid peroxidation. Hyperlipemia in Mg-deficient rats was accompanied by increased oxidation of very-low-density lipoproteins and low-density lipoproteins. Moreover, very-low-density lipoproteins and high-density lipoproteins from Mg-deficient rats were more susceptible to oxidative damage following iron incubation. Mg deficiency increased lipid peroxidation in liver, heart and skeletal muscles. Their homogenates were more susceptible to in vitro peroxidation. Mg deficiency has been discussed as a possible contributory factor in the development of cardiovascular disease and was associated with tissue damage and membrane alteration. These results demonstrate for the first time that Mg affects the susceptibility of lipoproteins to peroxidation and suggest that the mechanism responsible for the pathological consequences of Mg deficiency may be mediated by lipid peroxidation products.
将缺镁饮食和对照饮食分别喂给断乳的Wistar大鼠8天。通过连续制备性超速离心将血浆脂蛋白分离成不同的密度类别。在铁诱导脂质过氧化之前或之后，根据脂蛋白和组织匀浆中的硫代巴比妥酸反应性物质来测量脂质过氧化程度。缺镁大鼠的高脂血症伴随着极低密度脂蛋白和低密度脂蛋白氧化增加。此外，铁孵育后，缺镁大鼠的极低密度脂蛋白和高密度脂蛋白更容易受到氧化损伤。缺镁会增加肝脏、心脏和骨骼肌中的脂质过氧化。它们的匀浆在体外更易发生过氧化。缺镁已被认为是心血管疾病发展的一个可能因素，并且与组织损伤和膜改变有关。这些结果首次证明镁会影响脂蛋白对过氧化的敏感性，并表明缺镁病理后果的机制可能由脂质过氧化产物介导。
