Ikeda I, Imasato Y, Nagao H, Sasaki E, Sugano M, Imaizumi K, Yazawa K
Laboratory of Nutrition Chemistry, Kyushu University School of Agriculture, Fukuoka, Japan.
Life Sci. 1993;52(16):1371-9. doi: 10.1016/0024-3205(93)90172-y.
Lymphatic transport of docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids given in the forms of triglyceride, ethyl ester of free acid and their effect on cholesterol transport was compared in lymph-cannulated rats. Lymphatic recovery of DHA and EPA given by stomach tube in the form of triglyceride in which they were mainly located at the 2-position was significantly higher than that of the ethyl ester or free acid during the first 6 hr after the administration and the tendency continued until 9 hr. In contrast, the 9 to 24 hr recovery of DHA and EPA in the forms of ethyl ester and free acid was considerably higher than that of triglyceride. Consequently, cumulative 24 hr recovery of EPA was comparable among the three forms. However, the 24 hr recovery of DHA was highest in free acid, lowest in ethyl ester and intermediate in triglyceride. Recovery of the free acid between 9 and 24 hr after administration was significantly higher than that given in the forms of triglyceride or ethyl ester. Cholesterol recovery in lymph of rats given with ethyl ester or free acid was lower than that given with triglyceride at an early stage after the administration in both EPA and DHA. Cumulative 24 hr recovery of cholesterol in rats given these fatty acids as ethyl ester was significantly lower than in those given as the other two forms.
在淋巴管插管大鼠中，比较了以甘油三酯、游离酸乙酯形式给予的二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）的淋巴转运及其对胆固醇转运的影响。经胃管给予的甘油三酯形式的DHA和EPA（它们主要位于2位）在给药后的前6小时内，其淋巴回收率显著高于乙酯或游离酸形式，且这种趋势持续到9小时。相反，乙酯和游离酸形式的DHA和EPA在9至24小时的回收率明显高于甘油三酯形式。因此，EPA在三种形式下的24小时累积回收率相当。然而，DHA的24小时回收率以游离酸形式最高，乙酯形式最低，甘油三酯形式居中。给药后9至24小时游离酸的回收率明显高于甘油三酯或乙酯形式。在给药后的早期，给予乙酯或游离酸的大鼠淋巴中的胆固醇回收率低于给予甘油三酯的大鼠。给予这些脂肪酸乙酯形式的大鼠胆固醇的24小时累积回收率明显低于给予其他两种形式的大鼠。
