Kenny D
Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.
Scand J Clin Lab Invest Suppl. 1993;212:43-7.
A survey was undertaken through the Irish External Quality Assessment Scheme to investigate the extent to which acetoacetate and bilirubin affect routine methods in current use for measurement of serum creatinine. Spiking a sample with 10 mmol/l acetoacetate caused errors ranging from -28% to +22%. The greatest errors were found with end-point Jaffé methods and kinetic Jaffé methods in which there was no time delay between final reagent addition and start of absorbance measurement. A sample with high bilirubin concentration gave apparent creatinine results ranging from zero to 280 mumol/l. Although these interferences are well documented in the literature, this knowledge seems to have had little effect on methods in actual use.
通过爱尔兰外部质量评估计划进行了一项调查，以研究乙酰乙酸和胆红素对当前用于测量血清肌酐的常规方法的影响程度。向样品中加入10 mmol/l的乙酰乙酸会导致误差范围在-28%至+22%之间。在终点法Jaffé法和动力学Jaffé法中发现了最大误差，这两种方法在加入最终试剂和开始吸光度测量之间没有时间延迟。高胆红素浓度的样品给出的肌酐表观结果范围从零到280 μmol/l。尽管这些干扰在文献中有充分记载，但这些知识似乎对实际使用的方法影响甚微。
