Kondo S, Tamura A, Gomi S, Ikeda Y, Takeuchi T, Mitsuhashi S
Institute of Microbial Chemistry, Tokyo, Japan.
J Antibiot (Tokyo). 1993 Feb;46(2):310-5. doi: 10.7164/antibiotics.46.310.
Only a limited number of strains of methicillin-resistant Staphylococcus aureus (MRSA) moderately resistant to arbekacin (ABK) have been isolated clinically. Three inactivated products of ABK have been obtained by reaction with excess amounts of a crude enzyme preparation extracted from an ABK-resistant MRSA strain (MIC, 25 micrograms/ml). The 2"-O-phosphate was the major product together with small amounts of the 6'-N-acetate and the double modification product. The structures of these modification products were determined by MS and NMR spectral analyses.
临床上仅分离出少数对阿贝卡星（ABK）中度耐药的耐甲氧西林金黄色葡萄球菌（MRSA）菌株。通过与从一株耐ABK的MRSA菌株（最低抑菌浓度，25微克/毫升）中提取的粗酶制剂过量反应，获得了三种ABK的失活产物。2″-O-磷酸酯是主要产物，同时还有少量的6′-N-乙酸酯和双重修饰产物。通过质谱和核磁共振光谱分析确定了这些修饰产物的结构。
