Kim J W, Park J H, Park J W, Doh H J, Heo G S, Lee K J
Department of Clinical Pathology, Chungnam National University Hospital, Daejonsi, Korea.
Clin Chem. 1993 Apr;39(4):689-92.
The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samples from 38 patients with liver cirrhosis, nephrotic syndrome, or polyclonal gammopathy by capillary electrophoresis were done and the results were compared with those by conventional agarose gel electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All samples were analyzed in duplicate. We evaluated linearity of response, within-run CV, and the correlation between capillary electrophoresis and agarose gel electrophoresis.
研究了开放式管状毛细管电泳用于临床诊断的可能性。在一根未经处理的内径50微米×100厘米（至检测器65厘米）的毛细管中进行毛细管电泳，检测200纳米处的吸光度。确定了血清蛋白在不吸附到毛细管表面的情况下进行分离的条件。对38例肝硬化、肾病综合征或多克隆丙种球蛋白病患者的血清样本进行了毛细管电泳定量分析，并将结果与传统琼脂糖凝胶电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的结果进行比较。所有样本均进行了双份分析。我们评估了响应的线性、批内变异系数以及毛细管电泳和琼脂糖凝胶电泳之间的相关性。
