Nowak D, Pietras T, Antczak A, Król M, Piasecka G
Department of Pneumonology and Allergology, Medical University of Lódź, Poland.
Antonie Van Leeuwenhoek. 1993 Jan;63(1):77-83. doi: 10.1007/BF00871734.
The influence of lipopolysaccharide from Escherichia coli (LPS, 17 mg/kg body weight) on the lipid peroxidation process in organs of mice was studied. The content of conjugated dienes (CD), lipid peroxides (LP), malondialdehyde (MDA) (all three lipid peroxidation by-products), peroxidase (PO) activity and wet-to-dry weight ratio in lungs, heart, spleen, kidneys and liver were determined 1.5 h after intravenous injection of LPS. Animals observed at this time-point had reduced activity and decreased body temperature by about 2 degrees C, however, all analysed organs did not reveal any changes of wet-to-dry weight ratio comparing to organs from mice injected with sterile, pyrogen free 0.9% NaCl. Only extracts from heart and lungs showed significant increase in the tissue level of at least two lipid peroxidation products. The heart content of CD, MDA, and LP was about 1.5-, 1.3-, and 2.4-fold higher than in control group. In lungs CD and MDA increased 3.3- and 1.3-times but in spleen only content of LP was elevated. In these organs the suppression of PO activity was also observed. Liver and kidneys did not reveal any convincing enhancement of lipid peroxidation process and alterations of PO activity. Since free radical reactions are involved in lipid peroxidation process and inactivation of PO these results suggest that heart, lungs and spleen are the organs mostly exposed to oxidative stress during the first 1.5 h after single injection of LPS in mice.
研究了来自大肠杆菌的脂多糖（LPS，17毫克/千克体重）对小鼠器官脂质过氧化过程的影响。在静脉注射LPS 1.5小时后，测定肺、心脏、脾脏、肾脏和肝脏中共轭二烯（CD）、脂质过氧化物（LP）、丙二醛（MDA）（这三种均为脂质过氧化副产物）的含量、过氧化物酶（PO）活性以及湿重与干重之比。此时观察到的动物活动减少，体温下降约2摄氏度，然而，与注射无菌、无热原的0.9%氯化钠的小鼠的器官相比，所有分析的器官的湿重与干重之比均未显示任何变化。只有心脏和肺的提取物显示至少两种脂质过氧化产物的组织水平显著增加。心脏中CD、MDA和LP的含量分别比对照组高约1.5倍、1.3倍和2.4倍。肺中CD和MDA分别增加了3.3倍和1.3倍，但脾脏中仅LP含量升高。在这些器官中还观察到PO活性受到抑制。肝脏和肾脏未显示脂质过氧化过程有任何明显增强以及PO活性的改变。由于自由基反应参与脂质过氧化过程以及PO的失活，这些结果表明，在小鼠单次注射LPS后的最初1.5小时内，心脏、肺和脾脏是最易受到氧化应激影响的器官。
