Armstrong D N, Krenz H K, Modlin I M, Ballantyne G H
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06510.
Gut. 1993 Apr;34(4):483-8. doi: 10.1136/gut.34.4.483.
The effects of bile on small bowel motility were studied in isolated, perfused rabbit terminal ileum. It was proposed that bile delivery into the distal ileum would inhibit ileal motor activity, by peptide YY (PYY) release and therefore the effect of luminal bile on motor activity was examined and PYY release measured. Luminal bile and taurocheodeoxycholic acid (10 mmol) inhibited ileal motor activity. Arterial infusion of venous effluents from a bile inhibited ileum suppressed motor activity in a second isolated ileum. This shows the presence of a humoral inhibitor of ileal motor activity. Luminal bile increased venous PYY concentrations (42.5 (8.5) to 502 (46.2) pmol/l; p < 0.01) and increased bile salt values (1.7 (0.36) to 88.6 (5.6) 10 mumol/l/l; p < 0.005). Arterial infusion of taurocheodeoxycholic acid at concentrations found in the venous effluent (100 mumol/l/l) suppressed motility (p < 0.001) but infusion of PYY at concentrations in the venous effluent (500.0 pmol/l) failed to inhibit motility. Furthermore, PYY antagonist, PYX 1, failed to reverse the bile induced inhibition of motility. Luminal bile salts inhibit terminal ileal motility and this is independent of PYY release. By slowing motility, bile salts may participate in their own absorption by the 'ileal pump' and in the 'ileal brake' mechanism.
在离体灌注的兔回肠末端研究了胆汁对小肠运动的影响。有人提出胆汁进入回肠末端会通过肽YY（PYY）释放抑制回肠运动活性，因此检测了肠腔胆汁对运动活性的影响并测定了PYY释放。肠腔胆汁和牛磺鹅去氧胆酸（10 mmol）抑制回肠运动活性。从胆汁抑制的回肠动脉输注静脉流出液可抑制第二个离体回肠的运动活性。这表明存在回肠运动活性的体液抑制剂。肠腔胆汁增加静脉PYY浓度（从42.5（8.5）至502（46.2）pmol/l；p<0.01）并增加胆汁盐值（从1.7（0.36）至88.6（5.6）10μmol/l/l；p<0.005）。以静脉流出液中发现的浓度（100μmol/l/l）动脉输注牛磺鹅去氧胆酸可抑制运动（p<0.001），但以静脉流出液中的浓度（500.0 pmol/l）输注PYY未能抑制运动。此外，PYY拮抗剂PYX 1未能逆转胆汁诱导的运动抑制。肠腔胆汁盐抑制回肠末端运动，且这与PYY释放无关。通过减慢运动，胆汁盐可能通过“回肠泵”和“回肠制动”机制参与自身的吸收。
