Cui S, Chesson C, Hope R
Department of Genetics, University of Adelaide, South Australia.
Lab Anim. 1993 Apr;27(2):116-23. doi: 10.1258/002367793780810397.
The aim of this survey was to measure levels of genetic variation within and between 5 different strains of outbred Swiss mice. Ten to 15 animals from each strain (NIH, Q(S), ARC, IMVS and STUD) were typed, using allozyme electrophoresis, at 10 gene loci: Mod-1, Idh-1, Gpi-I, Es-1, Es-3, Hbb, Pep-3, Gr-1, Got-2 and Pgm-1. Polymorphic variation in at least one of the 5 strains was detected at all 10 loci. The proportion of polymorphic loci ranged from 0.3 (NIH) to 0.8 (IMVS) with a mean of 0.52. Average expected heterozygosities ranged from 0.08 (NIH) to 0.37 (IMVS) with a mean of 0.21. The inbred strain SWR was, as expected, homozygous at all 10 loci. The amount of allelic substitution between pairs of strains was quantified using Nei's genetic distance, and a dendrogram based on these genetic distances showed a close overall similarity in its branching pattern to the known genealogy of the strains. This survey showed that a considerable degree of genetic variation persists in the 5 strains examined, a level of variation similar to that previously detected by Rice and O'Brien (1980) in 3 other outbred Swiss strains.
本次调查的目的是测定5种不同品系的远交瑞士小鼠内部以及之间的遗传变异水平。使用等位酶电泳法，对每个品系（NIH、Q(S)、ARC、IMVS和STUD）的10至15只动物在10个基因位点进行分型：Mod-1、Idh-1、Gpi-I、Es-1、Es-3、Hbb、Pep-3、Gr-1、Got-2和Pgm-1。在所有10个位点均检测到至少一个品系存在多态性变异。多态性位点的比例范围为0.3（NIH）至0.8（IMVS），平均为0.52。平均预期杂合度范围为0.08（NIH）至0.37（IMVS），平均为0.21。正如预期的那样，近交系SWR在所有10个位点均为纯合子。使用Nei氏遗传距离对品系对之间的等位基因替代量进行了量化，基于这些遗传距离的树形图显示其分支模式与这些品系已知的谱系在总体上有密切的相似性。本次调查表明，在所检测的5个品系中存在相当程度的遗传变异，这一变异水平与Rice和O'Brien（1980年）之前在其他3个远交瑞士品系中检测到的水平相似。
