通过酶联免疫吸附测定、间接免疫荧光和病毒分离法检测鼻咽分泌物中的呼吸道合胞病毒：一项比较研究。
Detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study.
作者信息
Popow-Kraupp T, Kern G, Binder C, Tuma W, Kundi M, Kunz C
出版信息
J Med Virol. 1986 Jun;19(2):123-34. doi: 10.1002/jmv.1890190204.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of respiratory syncytial virus (RSV) antigens in nasopharyngeal secretions (NPS) from children with acute respiratory disease. Antisera against RSV nucleocapsids were used as immunoreagents for this test system. The results obtained by RSV antigen ELISA were compared to those of indirect immunofluorescence (IF) and tissue culture virus isolation (TC). Of the 404 NPS obtained, 278 were tested in parallel by ELISA and IF and 205 by ELISA and TC, and 89 were screened in parallel by all three methods. The sensitivity of ELISA in relation to IF was 86.7%, the specificity 95.7%. Sensitivity and specificity obtained by ELISA were 89.9% and 94.4%, respectively, compared to TC. False-negative results were obtained with all three test systems used.
开发了一种酶联免疫吸附测定（ELISA）方法，用于检测患有急性呼吸道疾病儿童的鼻咽分泌物（NPS）中的呼吸道合胞病毒（RSV）抗原。针对RSV核衣壳的抗血清用作该检测系统的免疫试剂。将RSV抗原ELISA获得的结果与间接免疫荧光（IF）和组织培养病毒分离（TC）的结果进行比较。在获得的404份NPS中，278份同时通过ELISA和IF进行检测，205份通过ELISA和TC进行检测，89份通过所有三种方法进行平行筛查。ELISA相对于IF的灵敏度为86.7%，特异性为95.7%。与TC相比，ELISA获得的灵敏度和特异性分别为89.9%和94.4%。使用的所有三种检测系统均出现了假阴性结果。
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