Miller S, Pesci E C, Pickett C L
Department of Microbiology and Immunology, Chandler Medical Center, University of Kentucky, Lexington 40536-0084.
Infect Immun. 1993 Jul;61(7):2930-6. doi: 10.1128/iai.61.7.2930-2936.1993.
A Campylobacter jejuni homolog of the lcrD/flbF family of genes was cloned and sequenced. The nucleotide sequence of the gene, called flbA, predicted a protein of 78,864 Da, with significant homology to a group of related proteins including the Yersinia pestis LcrD, Salmonella typhimurium InvA, and Caulobacter crescentus FlbF proteins. The greatest homology was seen with the C. crescentus FlbF protein, with an overall amino acid sequence homology of 57%. An insertion mutation in the C. jejuni 81-176 flbA gene was constructed. The resultant strain did not synthesize flagellin and was nonmotile.
克隆并测序了空肠弯曲菌中lcrD/flbF基因家族的一个同源基因。该基因（称为flbA）的核苷酸序列预测编码一个78,864 Da的蛋白质，与一组相关蛋白质具有显著同源性，包括鼠疫耶尔森菌LcrD、鼠伤寒沙门氏菌InvA和新月柄杆菌FlbF蛋白。与新月柄杆菌FlbF蛋白的同源性最高，整体氨基酸序列同源性为57%。构建了空肠弯曲菌81-176 flbA基因的插入突变体。所得菌株不合成鞭毛蛋白且无运动性。
