McConnell E E
Ann Occup Hyg. 1995 Oct;39(5):727-35. doi: 10.1016/0003-4878(95)00038-g.
Several methods have been proposed to ascertain the potential toxicity of man-made vitreous fibres (MMVF) in animals. The most frequently used in vivo methods include inhalation (IH) (whole body and nose-only), intratracheal (IT) instillation, intrapleural injection-implantation and intraperitoneal (IP) injection. This report compares reports of studies using these methods in terms of their: (1) relevance to humans; (2) standardization of technique; (3) validation of method; (4) need for fibre preparation; (5) estimation of maximum tolerated dose; (6) determination of 'overload'; (7) exposure regimen; (8) pathology requirements; (9) quality control procedures; (10) extent and type of peer review; and (11) value of data for risk assessment. The results of this investigation showed that the inhalation method was clearly superior to the other exposure methods in all respects, although it is the most expensive. The intratracheal instillation method was considered a second choice, while injection-implantation methods were fraught with scientific and practical problems and the data derived from studies using these techniques were considered of value only for the study of specific mechanistic issues.
已经提出了几种方法来确定人造玻璃纤维（MMVF）在动物体内的潜在毒性。最常用的体内方法包括吸入（IH）（全身吸入和仅经鼻吸入）、气管内（IT）滴注、胸膜内注射植入和腹腔内（IP）注射。本报告比较了使用这些方法的研究报告在以下方面的情况：（1）与人类的相关性；（2）技术的标准化；（3）方法的验证；（4）纤维制备的需求；（5）最大耐受剂量的估计；（6）“过载”的确定；（7）暴露方案；（8）病理学要求；（9）质量控制程序；（10）同行评审的范围和类型；以及（11）风险评估数据的价值。这项调查的结果表明，吸入法在各方面显然优于其他暴露方法，尽管它是最昂贵的。气管内滴注法被认为是第二选择，而注射植入法充满了科学和实际问题，并且使用这些技术的研究所获得的数据仅被认为对特定机制问题的研究有价值。
