通过聚合酶链反应（PCR）和限制性内切酶分析将单核细胞增生李斯特菌4b血清型菌株分为两组。
Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis.
作者信息
Ericsson H, Stålhandske P, Danielsson-Tham M L, Bannerman E, Bille J, Jacquet C, Rocourt J, Tham W
机构信息
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):3872-4. doi: 10.1128/aem.61.11.3872-3874.1995.
Abstract
Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated. A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively. The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.
摘要
总共对133株单核细胞增生李斯特菌4b血清型菌株进行了研究。利用聚合酶链反应（PCR）技术扩增出一段2916 bp的片段，该片段包含单核细胞增生李斯特菌中两个基因inlA和inlB的部分序列。将获得的PCR产物用限制性内切酶AluI进行切割，通过凝胶电泳分离产生的片段，从而得到两个不同的组：PCR-限制性酶切分析I组和II组，分别包含37株和96株菌株。本文所述的PCR-限制性酶切分析方法可能是单核细胞增生李斯特菌4b血清型菌株分型的一种有用工具。
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