Park K B, Eun H C
Department of Dermatology, Samsung Medical Center, Seoul, South Korea.
J Dermatol Sci. 1995 Sep;10(2):159-65. doi: 10.1016/0923-1811(95)00433-s.
Irritant contact dermatitis is a common clinical problem. Primary irritation can be easily recognized, but cumulative irritation by daily exposure is hard to be diagnosed and the condition may fail to be clear even away from work. The mechanism of irritant dermatitis produced by repeated or combined exposure to clinical or subclinical doses of irritants is still poorly understood. In order to find out whether the subclinical doses of irritants affect each other by repeated or combined exposure according to their concentrations, non-invasive measurements, transepidermal water loss and laser Doppler flowmetry were used. Sodium lauryl sulfate, sodium hydroxide and benzalkonium chloride were serially diluted and patch-tested with large Finn chambers on Scanpor tape on the back of normal human volunteers and responses were followed up for 7 days. Twice repeated exposure with subclinical doses of irritants at 1 day intervals were also performed. Repeated daily applications for 5 days with subclinical doses of single or premixed irritants were performed to know the combined irritating effect. The irritant response was well correlated to the concentration of the irritants. However, increased response was not observed when subclinical doses were rechallenged on the previously patch tested sites. Twice-repeated exposure of subclinical doses of irritants increased skin irritancy when measured by transepidermal water loss and laser Doppler flowmetry. Some correlation and some discrepancies were observed between different evaluation methods in combined and repeated application tests with irritants of subclinical doses. Responses of skin irritancy induced by subclinical doses showed somewhat different pattern from that given strong irritants.(ABSTRACT TRUNCATED AT 250 WORDS)
刺激性接触性皮炎是一个常见的临床问题。原发性刺激易于识别，但日常接触引起的累积性刺激难以诊断，即使脱离工作环境病情也可能无法消除。对于反复或联合接触临床或亚临床剂量的刺激物所产生的刺激性皮炎的机制，目前仍了解甚少。为了弄清楚亚临床剂量的刺激物是否会根据其浓度通过反复或联合接触而相互影响，采用了非侵入性测量方法，即经皮水分流失和激光多普勒血流仪。将十二烷基硫酸钠、氢氧化钠和苯扎氯铵进行系列稀释，然后用大芬氏小室贴敷在正常人类志愿者背部的Scanpor胶带上进行斑贴试验，并对反应进行7天的随访。还进行了以1天为间隔对亚临床剂量的刺激物进行两次重复接触的试验。为了解联合刺激作用，对亚临床剂量的单一刺激物或预混刺激物进行了为期5天的每日重复涂抹试验。刺激反应与刺激物的浓度密切相关。然而，在先前进行斑贴试验的部位再次接触亚临床剂量时，未观察到反应增强。当通过经皮水分流失和激光多普勒血流仪测量时，亚临床剂量的刺激物两次重复接触会增加皮肤刺激性。在亚临床剂量刺激物的联合和重复应用试验中，不同评估方法之间观察到了一些相关性和一些差异。亚临床剂量引起的皮肤刺激反应模式与强刺激物引起的反应模式略有不同。（摘要截选至250词）
