Evangelou A, Kalfakakou V, Benveniste J, Arnoux B
Laboratory of Experimental Physiology, Faculty of Medicine, University of Ioannina, Greece.
Biol Trace Elem Res. 1995 Oct;50(1):43-55. doi: 10.1007/BF02789148.
PAF-acether is a phospholipid synthesized by most animal tissues and exerting a strong decrease on the heart's contractile force and coronary flow. PAF-acether (10(-9) and 10(-10)M) was administered to isolated guinea pig hearts perfused via the Langendorff apparatus with Chenoweth solution. Zinc (1.5 microM) is known to benefit heart function thus, Zn2+ (1.5, 7.5, and 30 microM) was added in the perfusing solution before or after PAF-acether administration. Contractile force, coronary flow, and heart rate were recorded by means of a Narco MK-IV Physiograph throughout all modes of perfusion. Calcium inhibitor (Verapamil 10(-10)M) and Pb+2 Co2+ (1.5 x 10(-6)M) were used subsequently in the perfusing solutions in order to elucidate some of the Zn and PAF interactions observed. All hearts were analyzed for their Zn and Ca content by means of an Atomic Absorption Spectrophotometry (AAS). Our data suggest that low concentrations of zinc (1.5 microM) can strongly inhibit PAF-induced decrease of contractile force and coronary flow. Zinc-inhibiting effects on PAF's negative inotropic action (myocytic level) is not exerted through Zn-Ca antagonism. Nevertheless, a Zn-Ca antagonism in the arteriolar level cannot be excluded. Zinc inhibits PAF selectively only if it is administered before PAF injection and this strongly suggests a receptor interaction between the metal and the phospholipid at the heart level.
血小板活化因子（PAF - 乙酰醚）是一种由大多数动物组织合成的磷脂，它会使心脏收缩力和冠状动脉血流量大幅下降。将PAF - 乙酰醚（10⁻⁹和10⁻¹⁰M）施用于通过Langendorff装置用Chenoweth溶液灌注的离体豚鼠心脏。已知锌（1.5微摩尔）对心脏功能有益，因此，在施用PAF - 乙酰醚之前或之后，在灌注溶液中加入Zn²⁺（1.5、7.5和30微摩尔）。在所有灌注模式下，通过Narco MK - IV生理记录仪记录收缩力、冠状动脉血流量和心率。随后在灌注溶液中使用钙抑制剂（维拉帕米10⁻¹⁰M）和Pb²⁺、Co²⁺（1.5×10⁻⁶M），以阐明所观察到的一些锌与PAF的相互作用。通过原子吸收分光光度法（AAS）分析所有心脏的锌和钙含量。我们的数据表明，低浓度的锌（1.5微摩尔）可以强烈抑制PAF诱导的收缩力和冠状动脉血流量的下降。锌对PAF负性肌力作用（心肌细胞水平）的抑制作用不是通过锌 - 钙拮抗作用发挥的。然而，不能排除在小动脉水平存在锌 - 钙拮抗作用。锌仅在PAF注射前给药时才选择性地抑制PAF，这强烈表明在心脏水平金属与磷脂之间存在受体相互作用。
