Brick D J, Brumlik M J, Buckley J T, Cao J X, Davies P C, Misra S, Tranbarger T J, Upton C
Department of Biochemistry and Microbiology, University of Victoria, B.C. Canada.
FEBS Lett. 1995 Dec 27;377(3):475-80. doi: 10.1016/0014-5793(95)01405-5.
We have noted a striking similarity between the sequences of proteins in a novel family of lipases we recently reported [Upton, C. and Buckley, J. T. (1995) Trends Biol. Sci. 20, 178-9] and more than 120 sequences from the database of Expressed Sequence Tags (dbEST) which correspond to at least 30 unique genes from arabidopsis, rice and maize. A cDNA (Arab-1) corresponding to one of these sequences was isolated, sequenced and translated. There was significant similarity to sequences in the new lipase family over the entire open reading frame of Arab-1 and when expressed in E. coli, the gene product was lipolytic. Arab-1 and genes for some of the other plant proteins appear to be differentially expressed. They may play a role in the regulation of lipid metabolism during plant development.
我们注意到，在我们最近报道的一个新型脂肪酶家族的蛋白质序列[厄普顿，C.和巴克利，J.T.（1995年）《生物学趋势》20，178 - 179]与来自表达序列标签数据库（dbEST）的120多个序列之间存在显著相似性，这些序列对应于拟南芥、水稻和玉米中至少30个独特的基因。分离、测序并翻译了与这些序列之一对应的一个cDNA（Arab - 1）。在Arab - 1的整个开放阅读框中，它与新脂肪酶家族的序列有显著相似性，并且当在大肠杆菌中表达时，该基因产物具有脂解作用。Arab - 1和其他一些植物蛋白的基因似乎存在差异表达。它们可能在植物发育过程中脂质代谢的调节中发挥作用。
