利用单克隆抗体对肺炎溶血素进行功能分析。
Functional analysis of pneumolysin by use of monoclonal antibodies.
作者信息
de los Toyos J R, Méndez F J, Aparicio J F, Vázquez F, Del Mar García Suárez M, Fleites A, Hardisson C, Morgan P J, Andrew P W, Mitchell T J
机构信息
Facultad de Medicina, Universidad de Oviedo, Spain.
出版信息
Infect Immun. 1996 Feb;64(2):480-4. doi: 10.1128/iai.64.2.480-484.1996.
Abstract
We have produced a panel of monoclonal antibodies to pneumolysin, the membrane-damaging toxin from Streptococcus pneumoniae. We have used these antibodies to identify three regions of the toxin sequence that are involved in the lytic mechanism of this toxin. Two of these sites probably form the cell binding site of this toxin. Antibodies to the third site inhibit the lytic action of this toxin but not the binding of this toxin to cells. This site is engaged in the oligomerization process involved in the formation of pores in cell membranes. Two of these epitopes are also present in the related toxin perfringolysin O.
摘要
我们制备了一组针对肺炎链球菌溶血素（一种来自肺炎链球菌的膜损伤毒素）的单克隆抗体。我们利用这些抗体确定了毒素序列中与该毒素裂解机制相关的三个区域。其中两个位点可能构成了该毒素的细胞结合位点。针对第三个位点的抗体可抑制该毒素的裂解作用，但不影响该毒素与细胞的结合。这个位点参与了细胞膜上形成孔道的寡聚化过程。其中两个表位也存在于相关毒素产气荚膜梭菌溶血素O中。
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