Hilton D J, Zhang J G, Metcalf D, Alexander W S, Nicola N A, Willson T A
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):497-501. doi: 10.1073/pnas.93.1.497.
Interleukins 4 (IL-4) and 13 (IL-13) have been found previously to share receptor components on some cells, as revealed by receptor cross-competition studies. In the present study, the cloning is described of murine NR4, a previously unrecognized receptor identified on the basis of sequence similarity with members of the hemopoietin receptor family. mRNA encoding NR4 was found in a wide range of murine cells and tissues. By using transient expression in COS-7 cells, NR4 was found to encode the IL-13 receptor alpha chain, a low-affinity receptor capable of binding IL-13 but not IL-4 or interleukins 2, -7, -9, or -15. Stable expression of the IL-13 receptor alpha chain (NR4) in CTLL-2 cells resulted in the generation of high-affinity IL-13 receptors capable of transducing a proliferative signal in response to IL-13 and, moreover, led to competitive cross-reactivity in the binding of IL-4 and IL-13. These results suggest that the IL-13 receptor alpha chain (NR4) is the primary binding subunit of the IL-13 receptor and may also be a component of IL-4 receptors.
先前通过受体交叉竞争研究发现，白细胞介素4（IL-4）和13（IL-13）在某些细胞上共享受体成分。在本研究中，描述了小鼠NR4的克隆，它是基于与造血因子受体家族成员的序列相似性而鉴定出的一种先前未被识别的受体。在多种小鼠细胞和组织中发现了编码NR4的mRNA。通过在COS-7细胞中进行瞬时表达，发现NR4编码IL-13受体α链，这是一种低亲和力受体，能够结合IL-13，但不能结合IL-4或白细胞介素2、-7、-9或-15。IL-13受体α链（NR4）在CTLL-2细胞中的稳定表达导致产生了高亲和力的IL-13受体，该受体能够转导对IL-13作出反应的增殖信号，而且还导致了IL-4和IL-13结合中的竞争性交叉反应。这些结果表明，IL-13受体α链（NR4）是IL-13受体的主要结合亚基，也可能是IL-4受体的一个成分。
