Lecomte J, Berthiaume L, Boudreault A
J Clin Microbiol. 1979 Jan;9(1):128-33. doi: 10.1128/jcm.9.1.128-133.1979.
Counterimmunoelectrophoresis with an antiserum raised in rabbits against the M protein of the avian N virus proved to be particularly useful for large-scale identification of influenza A virus isolates. Of a total of 231 hemagglutinating agents isolated from 1,656 rectal swabs collected from shore and open-country birds, 158 could be identified as influenza A viruses by counterimmunoelectrophoresis, and 75 were serologically related to Newcastle disease virus by hemagglutination inhibition with an antiserum to Newcastle disease virus. Two isolates contained a mixture of influenza A virus and Newcastle disease virus; although the Newcastle disease virus virus particles outnumbered the influenza A virus particles in a ratio of 1,000:1, as seen by electron microscopy, the latter could be readily detected by counterimmunoelectrophoresis. This type of assay appears to be of potential use for epidemiological surveillance of influenza virus isolated from humans and animals. It combines specificity, sensitivity, and simplicity.
用兔抗禽N病毒M蛋白产生的抗血清进行对流免疫电泳，结果证明对流免疫电泳对甲型流感病毒分离株的大规模鉴定特别有用。从岸边和野外鸟类采集的1656份直肠拭子中分离出231种血凝剂，其中158种可通过对流免疫电泳鉴定为甲型流感病毒，75种通过用新城疫病毒抗血清进行血凝抑制试验，血清学上与新城疫病毒相关。两种分离物含有甲型流感病毒和新城疫病毒的混合物；尽管通过电子显微镜观察，新城疫病毒颗粒与甲型流感病毒颗粒的数量比为1000:1，但后者可通过对流免疫电泳很容易地检测到。这种检测方法似乎对从人和动物中分离出的流感病毒的流行病学监测有潜在用途。它兼具特异性、敏感性和简便性。
