血液抗氧化酶作为煤矿工人接触或影响的标志物。
Blood antioxidant enzymes as markers of exposure or effect in coal miners.
作者信息
Perrin-Nadif R, Auburtin G, Dusch M, Porcher J M, Mur J M
机构信息
INSERM U 420, Faculté de Médecine, Vandoeuvre-lès-Nancy, France.
出版信息
Occup Environ Med. 1996 Jan;53(1):41-5. doi: 10.1136/oem.53.1.41.
OBJECTIVE
To investigate if blood Cu++/Zn++ superoxide dismutase, glutathione peroxidase, catalase, and total plasma antioxidant activities could be markers of biological activity resulting from exposure to respirable coal mine dust in active miners, and of pneumoconiosis in retired miners.
METHODS
Blood samples were randomly obtained from active surface workers (n = 30) and underground miners (n = 34), and from retired miners without (n = 21), and with (n = 33) pneumoconiosis. Antioxidant enzyme activities and total plasma antioxidants were measured in erythrocytes and plasma. Non-parametric tests were completed by analyses of covariance to compare antioxidants between groups, taking into account potential confounding factors (age, smoking history (pack-years)).
RESULTS
Erythrocyte Cu++/Zn++ superoxide dismutase activity was significantly higher in the group of underground miners than the group of surface workers. The differences in total plasma antioxidants and plasma glutathione peroxidase activity between both groups were related to age. Glutathione peroxidase activity increased in the plasma of retired miners with pneumoconiosis, compared with retired miners without pneumoconiosis. No differences were found either in erythrocyte antioxidant enzyme activities or in total plasma antioxidants between the groups of retired miners without and with pneumoconiosis.
CONCLUSIONS
In this study, erythrocyte Cu++/Zn++ superoxide dismutase activity may be considered as a marker of effect of respirable coal mine dust in exposed workers. This result is in agreement with the hypothesis that reactive oxygen species are involved in cell injury induced by coal mine dust, and may be predictive of the degree of inflammation and pneumoconiosis induced by coal mine dust. The increase in glutathione peroxidase activity in the plasma of retired miners with pneumoconiosis may be the result of a response to the increasing hydrogen peroxide (H2O2) production due to the disease process.
目的
研究血液中铜离子/锌离子超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶以及血浆总抗氧化活性是否可作为在职矿工接触可吸入煤矿粉尘所产生生物活性的标志物，以及退休矿工尘肺病的标志物。
方法
从在职露天工人（n = 30）和井下矿工（n = 34）中随机采集血样，同时从无（n = 21）尘肺病和有（n = 33）尘肺病的退休矿工中采集血样。测定红细胞和血浆中的抗氧化酶活性及血浆总抗氧化剂水平。通过协方差分析完成非参数检验，以比较各组之间的抗氧化剂水平，同时考虑潜在的混杂因素（年龄、吸烟史（包年数））。
结果
井下矿工组红细胞铜离子/锌离子超氧化物歧化酶活性显著高于露天工人组。两组间血浆总抗氧化剂和血浆谷胱甘肽过氧化物酶活性的差异与年龄有关。与无尘肺病的退休矿工相比，患有尘肺病的退休矿工血浆中谷胱甘肽过氧化物酶活性升高。在无尘肺病和患有尘肺病的退休矿工组之间，红细胞抗氧化酶活性和血浆总抗氧化剂水平均未发现差异。
结论
在本研究中，红细胞铜离子/锌离子超氧化物歧化酶活性可被视为接触可吸入煤矿粉尘的工人受影响的标志物。这一结果与活性氧参与煤矿粉尘诱导的细胞损伤这一假说相符，并且可能预测煤矿粉尘诱导的炎症程度和尘肺病。患有尘肺病的退休矿工血浆中谷胱甘肽过氧化物酶活性升高可能是对疾病过程中过氧化氢（H2O2）生成增加的一种反应。
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