Barry A L, Brown S D, Novick W J
Clinical Microbiology Institute, Tualatin, Oregon 97062, USA.
Eur J Clin Microbiol Infect Dis. 1995 Aug;14(8):724-6. doi: 10.1007/BF01690885.
In vitro susceptibility tests were performed with 350 selected strains of Streptococcus pneumoniae to evaluate disk diffusion tests with 30 micrograms and 1 microgram cefotaxime disks. Zones were compared to MICs of cefotaxime with and without its desacetyl metabolite. Cefotaxime was two to eight times more active than desacetyl cefotaxime, but the two compounds were additive when combined in vitro. For 30 micrograms disks, zone size breakpoints were < or = 27 mm, 28-30 mm and > or = 31 mm for resistant, intermediate and susceptible, respectively. For 1 microgram disks, those zone size criteria were reduced to < or = 13 mm, 14-16 mm and > or = 17 mm. The 30 micrograms disk that is currently available for testing other species can be used for testing pneumococci; however, the 1 microgram disk has some important advantages.
对350株选定的肺炎链球菌进行了体外药敏试验，以评估使用30微克和1微克头孢噻肟纸片的纸片扩散试验。将抑菌圈与有和没有去乙酰代谢物的头孢噻肟的最低抑菌浓度（MIC）进行比较。头孢噻肟的活性比去乙酰头孢噻肟高两到八倍，但这两种化合物在体外联合使用时具有相加作用。对于30微克的纸片，抑菌圈大小的折点分别为耐药时≤27毫米、中介时28 - 30毫米和敏感时≥31毫米。对于1微克的纸片，这些抑菌圈大小标准分别降至≤13毫米、14 - 16毫米和≥17毫米。目前可用于检测其他菌种的30微克纸片可用于检测肺炎球菌；然而，1微克的纸片有一些重要优势。
