Clements S, Cooke I D, Barratt C L
Department of Obstetrics and Gynaecology, Jessop Hospital for Women, Sheffield, UK.
Hum Reprod. 1995 Aug;10(8):2096-106. doi: 10.1093/oxfordjournals.humrep.a136242.
Comprehensive quality control procedures were integrated into the routine semen analysis workload of a large university-based andrology laboratory. Methods were chosen to match as far as possible those which have been used successfully for many years in disciplines such as clinical chemistry. Levey-Jennings and cusum charts were plotted in order to monitor the immunobead-binding test for antisperm antibodies and a video-taped control sample for computerized semen analysis. A cryopreserved semen control was also charted. Daily manual sperm counts were plotted against the corresponding computer-assisted semen analysis (CASA) value. Multiple readings of 30 slides were used to monitor morphology assessments. Monthly means for morphology were also calculated regularly. Coefficients of variation were calculated for all variables and were found to be more appropriate for some aspects, such as CASA, than for others, such as morphology, when difference from the previous reading of the same slide was found to be more useful. These integrated quality control procedures had a direct influence on the production of results from the laboratory. Together with a high standard of technician training, comprehensive routine quality control based on repeated analyses of control samples is an effective way of assuring the validity of semen analysis results.
综合质量控制程序被纳入一所大型大学男科实验室的常规精液分析工作流程中。所选用的方法尽可能与临床化学等学科中已成功使用多年的方法相匹配。绘制了Levey-Jennings图和累积和控制图，以监测抗精子抗体的免疫珠结合试验以及计算机化精液分析的录像对照样本。还绘制了一份冷冻保存精液对照样本。将每日手工精子计数与相应的计算机辅助精液分析（CASA）值进行对比。使用对30张载玻片的多次读数来监测形态学评估。还定期计算每月形态学均值。计算了所有变量的变异系数，发现对于某些方面（如CASA）比其他方面（如形态学）更适用，当发现与同一张载玻片的先前读数存在差异时，这种差异更为有用。这些综合质量控制程序对实验室结果的产生有直接影响。与高标准的技术人员培训相结合，基于对对照样本进行重复分析的全面常规质量控制是确保精液分析结果有效性的有效方法。
