从亚美尼亚血红扇头蜱中分离出的立克次氏体菌株S的基因组和蛋白质特征分析
Genomic and proteinic characterization of strain S, a rickettsia isolated from Rhipicephalus sanguineus ticks in Armenia.
作者信息
Eremeeva M, Balayeva N, Roux V, Ignatovich V, Kotsinjan M, Raoult D
机构信息
Unité des Rickettsies, Centre National de la Recherche Scientifique EP J0054, Faculté de Médecine, Marseille, France.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2738-44. doi: 10.1128/jcm.33.10.2738-2744.1995.
Abstract
Strain S, a spotted fever group (SFG) rickettsia isolated from Rhipicephalus sanguineus ticks collected in Armenia, was identified. Microimmunofluorescence, sodium dodecyl sulfate-polyacrylamide gel protein electrophoresis and Western immunoblotting, PCR and then restriction fragment length polymorphism analysis, pulsed-field gel electrophoresis, and 16S rRNA gene sequencing were used to compare strain S with reference isolates. Strain S was found to possess proteinic, antigenic, and genomic patterns which were unique among SFG rickettsiae. Strain S is characterized by its high degree of pathogenicity for experimental animals, but its role as a potential human pathogen should be determined. The role of R. sanguineus ticks in the epidemiology of SFG rickettsiae is discussed.
摘要
从亚美尼亚采集的血红扇头蜱中分离出一种斑点热群（SFG）立克次体菌株S，并对其进行了鉴定。运用微量免疫荧光法、十二烷基硫酸钠 - 聚丙烯酰胺凝胶蛋白质电泳和蛋白质免疫印迹法、聚合酶链反应以及随后的限制性片段长度多态性分析、脉冲场凝胶电泳和16S rRNA基因测序，将菌株S与参考分离株进行比较。结果发现菌株S具有在SFG立克次体中独一无二的蛋白质、抗原和基因组模式。菌株S的特点是对实验动物具有高度致病性，但其作为潜在人类病原体的作用尚待确定。文中还讨论了血红扇头蜱在SFG立克次体流行病学中的作用。
相似文献
1
Genomic and proteinic characterization of strain S, a rickettsia isolated from Rhipicephalus sanguineus ticks in Armenia.从亚美尼亚血红扇头蜱中分离出的立克次氏体菌株S的基因组和蛋白质特征分析
J Clin Microbiol. 1995 Oct;33(10):2738-44. doi: 10.1128/jcm.33.10.2738-2744.1995.
2
Rickettsia aeschlimannii sp. nov., a new spotted fever group rickettsia associated with Hyalomma marginatum ticks.埃氏立克次体新种，一种与边缘璃眼蜱相关的新型斑点热群立克次体。
Int J Syst Bacteriol. 1997 Apr;47(2):548-54. doi: 10.1099/00207713-47-2-548.
3
Rickettsia africae sp. nov., the etiological agent of African tick bite fever.非洲立克次体新种，非洲蜱咬热的病原体。
Int J Syst Bacteriol. 1996 Apr;46(2):611-4. doi: 10.1099/00207713-46-2-611.
4
Confirmation that Rickettsia helvetica sp. nov. is a distinct species of the spotted fever group of rickettsiae.瑞士立克次氏体新种（Rickettsia helvetica sp. nov.）是斑点热群立克次氏体中一个独特物种的确认。
Int J Syst Bacteriol. 1993 Jul;43(3):521-6. doi: 10.1099/00207713-43-3-521.
5
Rickettsia slovaca sp. nov., a member of the spotted fever group rickettsiae.斯洛伐克立克次体新种，斑点热群立克次体的一个成员。
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1455-62. doi: 10.1099/00207713-48-4-1455.
6
Proteinic and genomic identification of spotted fever group rickettsiae isolated in the former USSR.前苏联分离出的斑点热群立克次体的蛋白质组学和基因组学鉴定
J Clin Microbiol. 1993 Oct;31(10):2625-33. doi: 10.1128/jcm.31.10.2625-2633.1993.
7
Isolation and identification of a rickettsial strain related to Rickettsia massiliae in Greek ticks.希腊蜱中一株与马赛立克次体相关的立克次体菌株的分离与鉴定。
Am J Trop Med Hyg. 1994 Mar;50(3):365-72. doi: 10.4269/ajtmh.1994.50.365.
8
A new pathogenic spotted fever group rickettsia from Africa.一种来自非洲的新型致病性斑点热群立克次体。
J Trop Med Hyg. 1994 Jun;97(3):129-37.
9
Phenotypic and genotypic characterization of spotted fever group Rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks.从加泰罗尼亚血红扇头蜱分离出的斑点热群立克次体的表型和基因型特征
J Clin Microbiol. 1996 Nov;34(11):2688-94. doi: 10.1128/jcm.34.11.2688-2694.1996.
10
Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks.阿斯特拉罕热立克次体：从人类和微小扇头蜱分离株的抗原性和基因型分析
Am J Trop Med Hyg. 1994 Nov;51(5):697-706. doi: 10.4269/ajtmh.1994.51.697.
引用本文的文献
1
Diversity and Distribution of Bacterial and Parasitic Tick-Borne Pathogens in Armenia, Transcaucasia.亚美尼亚外高加索地区蜱传细菌和寄生虫病原体的多样性与分布
Iran J Public Health. 2024 Nov;53(11):2563-2571. doi: 10.18502/ijph.v53i11.16960.
2
A retrospective chart review study to describe selected zoonotic and arboviral etiologies in hospitalized febrile patients in the Republic of Armenia.一项回顾性病历审查研究，旨在描述亚美尼亚共和国住院发热患者中选定的人畜共患病和虫媒病毒病因。
BMC Infect Dis. 2016 Aug 24;16(1):445. doi: 10.1186/s12879-016-1764-z.
3
Evidence of Rickettsia and Orientia Infections Among Abattoir Workers in Djibouti.吉布提屠宰场工人中存在立克次体和东方体感染的证据。
Am J Trop Med Hyg. 2016 Aug 3;95(2):462-5. doi: 10.4269/ajtmh.15-0775. Epub 2016 Jun 6.
4
Eschar-associated spotted fever rickettsiosis, Bahia, Brazil.巴西巴伊亚的焦痂伴随性斑点热立克次体病。
Emerg Infect Dis. 2011 Feb;17(2):275-8. doi: 10.3201/eid1702.100859.
5
Tick-borne rickettsioses around the world: emerging diseases challenging old concepts.全球蜱传立克次体病：挑战旧有观念的新兴疾病
Clin Microbiol Rev. 2005 Oct;18(4):719-56. doi: 10.1128/CMR.18.4.719-756.2005.
6
Gene sequence-based criteria for identification of new rickettsia isolates and description of Rickettsia heilongjiangensis sp. nov.基于基因序列鉴定新型立克次氏体分离株及描述黑龙江立克次氏体新种的标准
J Clin Microbiol. 2003 Dec;41(12):5456-65. doi: 10.1128/JCM.41.12.5456-5465.2003.
7
Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review.蜱虫（蜱螨亚纲：硬蜱科）病原体DNA的分子检测：综述
Exp Appl Acarol. 1999 Dec;23(12):929-60. doi: 10.1023/a:1006313803979.
8
Taxonomic relationships among spotted fever group rickettsiae as revealed by antigenic analysis with monoclonal antibodies.利用单克隆抗体进行抗原分析揭示的斑点热群立克次体之间的分类关系。
J Clin Microbiol. 1998 Apr;36(4):887-96. doi: 10.1128/JCM.36.4.887-896.1998.
9
Distribution of immunogenic epitopes on the two major immunodominant proteins (rOmpA and rOmpB) of Rickettsia conorii among the other rickettsiae of the spotted fever group.康氏立克次体的两种主要免疫显性蛋白（rOmpA 和 rOmpB）上的免疫原性表位在斑点热群其他立克次体中的分布。
Clin Diagn Lab Immunol. 1997 Nov;4(6):753-63. doi: 10.1128/cdli.4.6.753-763.1997.
10
Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases.立克次体病的实验室诊断：诊断新旧立克次体病的当前方法
J Clin Microbiol. 1997 Nov;35(11):2715-27. doi: 10.1128/jcm.35.11.2715-2727.1997.
本文引用的文献
1
2
Phylogenetic analysis of the genus Rickettsia by 16S rDNA sequencing.基于16S rDNA测序的立克次氏体属系统发育分析。
Res Microbiol. 1995 Jun;146(5):385-96. doi: 10.1016/0923-2508(96)80284-1.
3
Mediterranean spotted fever in Marseille, France: correlation between prevalence of hospitalized patients, seroepidemiology, and prevalence of infected ticks in three different areas.法国马赛的地中海斑疹热：三个不同地区住院患者患病率、血清流行病学与感染蜱虫患病率之间的相关性
Am J Trop Med Hyg. 1993 Feb;48(2):249-56. doi: 10.4269/ajtmh.1993.48.249.
4
Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis.运用脉冲场凝胶电泳技术对斑点热群立克次体进行基因分型及系统发育分析。
J Bacteriol. 1993 Aug;175(15):4895-904. doi: 10.1128/jb.175.15.4895-4904.1993.
5
Genetic relatedness of Bordetella species as determined by macrorestriction digests resolved by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳解析的宏观限制性酶切分析确定的博德特氏菌属的遗传相关性。
Int J Syst Bacteriol. 1993 Oct;43(4):659-64. doi: 10.1099/00207713-43-4-659.
6
Ancestral divergence of Rickettsia bellii from the spotted fever and typhus groups of Rickettsia and antiquity of the genus Rickettsia.贝氏立克次氏体与斑点热群和斑疹伤寒群立克次氏体的祖先分歧及立克次氏体属的古老性。
Int J Syst Bacteriol. 1994 Oct;44(4):798-804. doi: 10.1099/00207713-44-4-798.
7
A new pathogenic spotted fever group rickettsia from Africa.一种来自非洲的新型致病性斑点热群立克次体。
J Trop Med Hyg. 1994 Jun;97(3):129-37.
8
Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA.通过分析聚合酶链反应扩增DNA的限制性片段长度多态性来区分斑点热群立克次体菌种。
J Clin Microbiol. 1994 Mar;32(3):803-10. doi: 10.1128/jcm.32.3.803-810.1994.
9
Isolation and identification of a rickettsial strain related to Rickettsia massiliae in Greek ticks.希腊蜱中一株与马赛立克次体相关的立克次体菌株的分离与鉴定。
Am J Trop Med Hyg. 1994 Mar;50(3):365-72. doi: 10.4269/ajtmh.1994.50.365.
10
Proteinic and genomic identification of spotted fever group rickettsiae isolated in the former USSR.前苏联分离出的斑点热群立克次体的蛋白质组学和基因组学鉴定
J Clin Microbiol. 1993 Oct;31(10):2625-33. doi: 10.1128/jcm.31.10.2625-2633.1993.
Zotero 插件